Cloned (Comment) | Organism |
---|---|
generation of a synthetic gene, cloning method overview, subcloning in Escherichia coli strain JM109, sequence comparison with wild-type gene, recombinant enzyme expression of the synthetic gene syn-mimosinase ORF in Escherichia coli strain BL21(DE3) pLysS | Leucaena leucocephala |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-Phe | competitive inhibition | Leucaena leucocephala | |
L-Trp | competitive inhibition | Leucaena leucocephala | |
L-Tyr | competitive inhibition | Leucaena leucocephala |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | the enzyme sequence contains a chloroplast transit peptide | Leucaena leucocephala | 9507 | - |
additional information | the enzyme sequence contains a 43 amino acid signal peptide | Leucaena leucocephala | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
mimosine + H2O | Leucaena leucocephala | - |
3-hydroxy-4-(1H)-pyridone + pyruvate + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leucaena leucocephala | A8CEI3 | collected from University of Hawaii research station, Waimanalo, Honolulu | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | mature | Leucaena leucocephala | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
mimosine + H2O | - |
Leucaena leucocephala | 3-hydroxy-4-(1H)-pyridone + pyruvate + NH3 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Leucaena leucocephala |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Leucaena leucocephala |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | dependent on | Leucaena leucocephala |
General Information | Comment | Organism |
---|---|---|
physiological function | mimosinase is an important enzyme especially in the context of metabolic engineering of plant secondary metabolite as it catalyzes the degradation of mimosine, which is a toxic secondary metabolite found in all Leucaena and Mimosa species | Leucaena leucocephala |