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Literature summary for 3.5.1.61 extracted from

  • Negi, V.S.; Borthakur, D.
    Heterologous expression and characterization of mimosinase from Leucaena leucocephala (2016), Methods Mol. Biol., 1405, 59-77 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
generation of a synthetic gene, cloning method overview, subcloning in Escherichia coli strain JM109, sequence comparison with wild-type gene, recombinant enzyme expression of the synthetic gene syn-mimosinase ORF in Escherichia coli strain BL21(DE3) pLysS Leucaena leucocephala

Inhibitors

Inhibitors Comment Organism Structure
L-Phe competitive inhibition Leucaena leucocephala
L-Trp competitive inhibition Leucaena leucocephala
L-Tyr competitive inhibition Leucaena leucocephala

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast the enzyme sequence contains a chloroplast transit peptide Leucaena leucocephala 9507
-
additional information the enzyme sequence contains a 43 amino acid signal peptide Leucaena leucocephala
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
mimosine + H2O Leucaena leucocephala
-
3-hydroxy-4-(1H)-pyridone + pyruvate + NH3
-
?

Organism

Organism UniProt Comment Textmining
Leucaena leucocephala A8CEI3 collected from University of Hawaii research station, Waimanalo, Honolulu
-

Source Tissue

Source Tissue Comment Organism Textmining
seed mature Leucaena leucocephala
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
mimosine + H2O
-
Leucaena leucocephala 3-hydroxy-4-(1H)-pyridone + pyruvate + NH3
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Leucaena leucocephala

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Leucaena leucocephala

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate dependent on Leucaena leucocephala

General Information

General Information Comment Organism
physiological function mimosinase is an important enzyme especially in the context of metabolic engineering of plant secondary metabolite as it catalyzes the degradation of mimosine, which is a toxic secondary metabolite found in all Leucaena and Mimosa species Leucaena leucocephala