Cloned (Comment) | Organism |
---|---|
gene NAAA, genotyping, six splice variants (a1, b1, c1, a2, b2, and c2), whereof four major splice variants (a1, a2, b2, and c2) are identified in a human prostate cancer cell line LNCaP, they are composed of exons 1-11, exons 1-10 and 12, exons 1-9 and 12, and exons 1-8 and 12, respectively, quantitative PCR expression analysis, method development for individual quantification. Variants a1 and a2 encode the same full-length NAAA protein, which is catalytically active, while b2 and c2 are translated to C-terminally truncated proteins. Recombinant expression in HEK-293 cells. The truncated forms are detected as catalytically inactive precursor proteins, but not as mature forms, expression of N-terminally FLAG-tagged isoform A of human NAAA, corresponding to splice variant a1, in HEK-293 cells. The mature enzyme form loses the N-terminal FLAG tag | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
L325A | site-directed mutagenesis of isozyme A, the mutant shows reduced activity compared to wild-type | Homo sapiens |
L325T | site-directed mutagenesis of isozyme A, the mutant shows reduced activity compared to wild-type | Homo sapiens |
T335A | site-directed mutagenesis of isozyme A, the mutant is almost inactive | Homo sapiens |
T335V | site-directed mutagenesis of isozyme A, the mutant is almost inactive | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
lysosome | - |
Homo sapiens | 5764 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
palmitoylethanolamide + H2O | Homo sapiens | - |
palmitate + ethanolamine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q02083 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
CMK cell | - |
Homo sapiens | - |
DU-145 cell | - |
Homo sapiens | - |
HEK-293 cell | - |
Homo sapiens | - |
HeLa cell | low expression level | Homo sapiens | - |
LNCaP cell | high expression level | Homo sapiens | - |
MCF-7 cell | - |
Homo sapiens | - |
additional information | all cell types examined express NAAA but its expression levels vary by cell type, expression patterns of splicing variants a1, a2, b2, and c2, overview. Splicing variants a1 and b2 show the highest and lowest expression levels, respectively, among the four variants in all of the prostate cells and other human cells | Homo sapiens | - |
PC-3 cell | - |
Homo sapiens | - |
prostate gland | - |
Homo sapiens | - |
prostate gland cancer cell | the total levels of NAAA mRNAs in androgen-sensitive cells like LNCaP are higher than those in androgen-insensitive cells | Homo sapiens | - |
THP-1 cell | high expression level | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
palmitoylethanolamide + H2O | - |
Homo sapiens | palmitate + ethanolamine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 26300, recombinant untagged isozyme A, SDS-PAGE, x * 24200, isozyme B, SDS-PAGE, x * 222000, isozyme C, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
N-acylethanolamine acid amidase | - |
Homo sapiens |
NAAA | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
additional information | residues Leu325 and Thr335 are catalytically important for isozyme A, and the latter one is indispensable for the enzyme activity of NAAA | Homo sapiens |