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Literature summary for 3.5.1.59 extracted from

  • Romao, M.J.; Turk, D.; Gomis-Ruth, F.X.; Huber, R.; Schuhmacher, G.; Möllering, H.; Russmann, L.
    Crystal structure analysis, refinement and enzymatic reaction mechanism of N-carbamoylsarcosine amidohydrolase from Arthrobacter sp. at 2.0 A resolution (1992), J. Mol. Biol., 226, 1111-1130.
    View publication on PubMed
Search for more literature for 3.5.1.59

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Arthrobacter sp.

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Arthrobacter sp.

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoic acid)
-
 Arthrobacter sp.
Hg-compounds
-
 Arthrobacter sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3
-
 N-carbamoylsarcosine
-
 Arthrobacter sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 4 * 35000, SDS-PAGE Arthrobacter sp.
120000
-
 HPLC gel filtration at pH 6 Arthrobacter sp.

Organism

Organism UniProt Comment Textmining
Arthrobacter sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
from recombinant Escherichia coli Arthrobacter sp.

Reaction

Reaction Comment Organism Reaction ID
N-carbamoylsarcosine + H2O = sarcosine + CO2 + NH3 tentative catalytic mechanism Arthrobacter sp.
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1
-
-
 Arthrobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-carbamoylsarcosine + H2O
-
 Arthrobacter sp. sarcosine + CO2 + NH3
-
 ir

Subunits

Subunits Comment Organism
tetramer 4 * 35000, SDS-PAGE Arthrobacter sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Arthrobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 Tris, HEPES or K4P2O7 buffer Arthrobacter sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 6.5 stability maximum, phosphate buffer Arthrobacter sp.