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Literature summary for 3.5.1.59 extracted from

  • Kim, J.M.; Shimizu, S.; Yamada, H.
    Purification and characterization of a novel enzyme, N-carbamoylsarcosine amidohydrolase, from Pseudomonas putida 77 (1986), J. Biol. Chem., 261, 11832-11839.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Pseudomonas putida

Inhibitors

Inhibitors Comment Organism Structure
Ag2+
-
Pseudomonas putida
Cu2+
-
Pseudomonas putida
Hg2+
-
Pseudomonas putida
additional information no inhibition by 2 mM MgCl2, FeSO4, CdCl2, MnCl2, BaCl2, FeCl3, CaCl2, PbCl2, DTT or 2-mercaptoethanol, 1 mM 1,10-phenanthroline, 2,2'-dipyridyl, hydrazine, phenylhydrazine, 8-hydroxyquinoline, 4 mM sarcosine, glycine, N-methyl-DL-valine, N-methyl-DL-alanine, N,N-dimethylglycine, N-methylleucine, methylurea, dimethylamine, trimethylamine or diethylamine Pseudomonas putida
NaN3 weak Pseudomonas putida
PCMB
-
Pseudomonas putida
Zn2+ weak Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2
-
N-carbamoylsarcosine
-
Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000
-
HPLC gel filtration Pseudomonas putida
88000
-
gel filtration Pseudomonas putida
102000
-
sedimentation equilibrium method Pseudomonas putida
27000
-
x * 27000, SDS-PAGE Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-carbamoylsarcosine + H2O Pseudomonas putida creatine pathway sarcosine + CO2 + NH3
-
ir

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
no glycoprotein
-
Pseudomonas putida

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.958
-
-
Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information poor substrates are N-carbamoyl derivatives of DL-tryptophan, DL-phenylalanine, 4-hydroxy-DL-phenylglycine, D-valine, 4-methyl-DL-phenylglycine, DL-2-amino-n-butyric acid, DL-threonine or D-leucine. No substrates are N-carbamoyl derivatives of L-glutamic acid, L-valine, L-tyrosine, L-isoleucine, L-histidine, L-asparagine, DL-aspartic acid, L-serine, DL-methionine or 2-aminoisobutyric acid Pseudomonas putida ?
-
?
N-carbamoylsarcosine + H2O best substrate Pseudomonas putida sarcosine + CO2 + NH3
-
ir
N-carbamoylsarcosine + H2O creatine pathway Pseudomonas putida sarcosine + CO2 + NH3
-
ir
N-methyl-N-carbamoyl-D-alanine + H2O hydrolysis at 13% the rate of N-carbamoylsarcosine Pseudomonas putida ?
-
?
N-carbamoylglycine + H2O hydrolysis at 10% the rate of N-carbamoylsarcosine Pseudomonas putida ?
-
?
N-carbamoyl-D-alanine + H2O hydrolysis at 7% the rate of N-carbamoylsarcosine Pseudomonas putida ?
-
?

Subunits

Subunits Comment Organism
? x * 27000, SDS-PAGE Pseudomonas putida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Pseudomonas putida

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 50 about half-maximal activity at 30°C and 50°C Pseudomonas putida

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
10 min, stable at pH 6-7 Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8
-
Pseudomonas putida

pH Range

pH Minimum pH Maximum Comment Organism
5 8.7 about half-maximal activity at pH 5 and 8.7 Pseudomonas putida

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 7 10 min, stable at 40°C Pseudomonas putida