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Literature summary for 3.5.1.58 extracted from

  • Matsumura, E.; Shin, T.; Murao, S.; Kawano, T.
    Substrate specificity and stoichiometry of Nalpha-benzyloxycarbonyl amino acid urethane hydrolase, from Streptococcus faecalis R ATCC 8043 (1985), Agric. Biol. Chem., 49, 973-979.
No PubMed abstract available
Search for more literature for 3.5.1.58

Application

Application Comment Organism
synthesis can be used in peptide synthesis procedures for removal of the protecting groups Z and Bz from Gly and Ala Enterococcus faecalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 N-benzyl-Gly
-
 Enterococcus faecalis
0.35
-
 N-benzyl-Ala
-
 Enterococcus faecalis
0.36
-
 N-Benzyloxycarbonyl-Gly
-
 Enterococcus faecalis
0.8
-
 N-Benzyloxycarbonyl-Ala
-
 Enterococcus faecalis

Organism

Organism UniProt Comment Textmining
Enterococcus faecalis
-
-
-
Enterococcus faecalis R / ATCC 8043
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 specific activity for 14 more substrates Enterococcus faecalis
0.0003
-
 for N-benzyloxycarbonyl-Ala Enterococcus faecalis
0.0007
-
 for N-benzyl-Ala Enterococcus faecalis
0.0067
-
 for N-benzyl-Gly Enterococcus faecalis
0.0074
-
 for N-benzyloxylcarbonyl-Gly Enterococcus faecalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information systematic examination on many more substituted amino acids Enterococcus faecalis ?
-
 ?
additional information systematic examination on many more substituted amino acids Enterococcus faecalis R / ATCC 8043 ?
-
 ?
N-(2-hydroxy)benzyl-Gly + H2O
-
 Enterococcus faecalis 2-hydroxybenzyl alcohol + Gly
-
 ?
N-(3-hydroxy)benzyl-Gly + H2O
-
 Enterococcus faecalis 3-hydroxybenzyl alcohol + Gly
-
 ?
N-(4-amino)benzyl-Gly + H2O
-
 Enterococcus faecalis 4-aminobenzyl alcohol + Gly
-
 ?
N-(4-hydroxy)benzyl-Gly + H2O
-
 Enterococcus faecalis 4-hydroxybenzyl alcohol + Gly
-
 ?
N-(4-methoxy)benzyloxycarbonyl-Gly + H2O
-
 Enterococcus faecalis 4-methoxybenzyl alcohol + CO2 + Gly
-
 ?
N-benzyl-Gly + H2O
-
 Enterococcus faecalis benzyl alcohol + Gly
-
 ?
N-benzyl-Gly + H2O
-
 Enterococcus faecalis R / ATCC 8043 benzyl alcohol + Gly
-
 ?
N-benzyl-Gly-Gly + H2O very poor substrate Enterococcus faecalis benzyl alcohol + Gly-Gly
-
 ?
N-benzyl-L-Ala + H2O
-
 Enterococcus faecalis benzyl alcohol + Ala
-
 ?
N-benzyloxycarbonyl-Gly + H2O
-
 Enterococcus faecalis benzyl alcohol + CO2 + Gly
-
 ?
N-benzyloxycarbonyl-Gly + H2O
-
 Enterococcus faecalis R / ATCC 8043 benzyl alcohol + CO2 + Gly
-
 ?
N-benzyloxycarbonyl-Gly-Gly + H2O very poor substrate Enterococcus faecalis benzyl alcohol + CO2 + Gly-Gly
-
 ?
N-benzyloxycarbonyl-Gly-Gly + H2O very poor substrate Enterococcus faecalis R / ATCC 8043 benzyl alcohol + CO2 + Gly-Gly
-
 ?
N-benzyloxycarbonyl-L-Ala + H2O
-
 Enterococcus faecalis benzyl alcohol + CO2 + Ala
-
 ?
N-benzyloxycarbonyl-L-Ala + H2O
-
 Enterococcus faecalis R / ATCC 8043 benzyl alcohol + CO2 + Ala
-
 ?