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Literature summary for 3.5.1.56 extracted from

  • Lu, X.; Wang, W.; Zhang, L.; Hu, H.; Xu, P.; Wei, T.; Tang, H.
    Molecular mechanism of N,N-dimethylformamide degradation in Methylobacterium sp. strain DM1 (2019), Appl. Environ. Microbiol., 85, e00275-19 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.56

Cloned(Commentary)

Cloned (Comment) Organism
encoded on plasmid pLVM1, DNA and amino acid sequence determination and analysis, genetic organization, sequence comparisons and phylogenetic analysis, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) Methylobacterium sp. DM1

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ slight inhibition Methylobacterium sp. DM1
Co2+ slight inhibition Methylobacterium sp. DM1
Cr2+ slight inhibition Methylobacterium sp. DM1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
27.6
-
 N,N-Dimethylformamide pH 6.5, 37°C, recombinant enzyme Methylobacterium sp. DM1

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ slight activation Methylobacterium sp. DM1
Fe2+ slight activation Methylobacterium sp. DM1

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
200000
-
 recombinant His6-tagged enzyme, native PAGE Methylobacterium sp. DM1

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N,N-dimethylformamide + H2O Methylobacterium sp. DM1
-
 dimethylamine + formate
-
 ?

Organism

Organism UniProt Comment Textmining
Methylobacterium sp. DM1 A0A2Y9CJ10
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Methylobacterium sp. DM1

Source Tissue

Source Tissue Comment Organism Textmining
additional information strain DM1 can utilize N,N-dimethylformamide, DMA, MA, methanol, ethanol, formamide, toluene, glycerol, and trisodium citrate as the sole source of carbon or carbon and nitrogen Methylobacterium sp. DM1
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N,N-dimethylformamide + H2O
-
 Methylobacterium sp. DM1 dimethylamine + formate
-
 ?

Subunits

Subunits Comment Organism
heterotetramer recombinent enzyme, alpha2beta2, 2 * 20000, alpha-subunit, + 2 * 80000, beta-subunit, SDS-PAGE Methylobacterium sp. DM1
More secondary structure analysis Methylobacterium sp. DM1

Synonyms

Synonyms Comment Organism
C0214_26885 locus name Methylobacterium sp. DM1
dmfA1
-
 Methylobacterium sp. DM1
dmfA2
-
 Methylobacterium sp. DM1
DMFase
-
 Methylobacterium sp. DM1
N,N-dimethylformamidase
-
 Methylobacterium sp. DM1

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 recombinant enzyme Methylobacterium sp. DM1

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 recombinant enzyme Methylobacterium sp. DM1

General Information

General Information Comment Organism
metabolism the enzyme is important and initial in the N,N-dimethylformamide (DMF) degradation pathway, transcriptional analysis of genes specific to DMF degradation, overview Methylobacterium sp. DM1
physiological function the enzyme is responsible for N,N-dimethylformamide degradation, molecular mechanism, overview Methylobacterium sp. DM1