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Literature summary for 3.5.1.56 extracted from

  • Schär, H.P.; Holzmann, W.; Ramos Tombo, G.M.; Ghisalba, O.
    Purification and characterization of N,N-dimethylformamidase from Pseudomonas DMF 3/3 (1986), Eur. J. Biochem., 158, 469-475.
    View publication on PubMed
Search for more literature for 3.5.1.56

Inhibitors

Inhibitors Comment Organism Structure
2,2'-dipyridyl
-
 Pseudomonas sp.
EDTA
-
 Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
13.8
-
 N,N-Dimethylformamide
-
 Pseudomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Fe iron protein Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
15000
-
 alpha2beta2, 2 * 15000 + 2 * 105000, SDS-PAGE Pseudomonas sp.
105000
-
 alpha2beta2, 2 * 15000 + 2 * 105000, SDS-PAGE Pseudomonas sp.
250000
-
 gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N,N-dimethylformamide + H2O Pseudomonas sp.
-
 dimethylamine + formate
-
 ?
N,N-dimethylformamide + H2O Pseudomonas sp. DMF 3/3
-
 dimethylamine + formate
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. DMF 3/3
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.98
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formamide + H2O 3.8% of the activity with N,N-dimethylformamide Pseudomonas sp. NH3 + formate
-
 ?
additional information less than 1% of the activity with N,N-dimethylformamide: acetamide, acrylamide, butyramide Pseudomonas sp. ?
-
 ?
additional information less than 1% of the activity with N,N-dimethylformamide: acetamide, acrylamide, butyramide Pseudomonas sp. DMF 3/3 ?
-
 ?
N,N-diethylformamide + H2O 2.6% of the activity with N,N-dimethylformamide Pseudomonas sp. diethylamine + formate
-
 ?
N,N-diethylformamide + H2O 2.6% of the activity with N,N-dimethylformamide Pseudomonas sp. DMF 3/3 diethylamine + formate
-
 ?
N,N-dimethylacetamide + H2O 3.4% of the activity with N,N-dimethylformamide Pseudomonas sp. dimethylamine + acetate
-
 ?
N,N-dimethylformamide + H2O
-
 Pseudomonas sp. dimethylamine + formate
-
 ?
N,N-dimethylformamide + H2O
-
 Pseudomonas sp. DMF 3/3 dimethylamine + formate
-
 ?
N-ethylformamide + H2O 31.5% of the activity with N,N-dimethylformamide Pseudomonas sp. ethylamine + formate
-
 ?
N-ethylformamide + H2O 31.5% of the activity with N,N-dimethylformamide Pseudomonas sp. DMF 3/3 ethylamine + formate
-
 ?
N-methylformamide + H2O 14.8% of the activity with N,N-dimethylformamide Pseudomonas sp. methylamine + formate
-
 ?
N-methylformamide + H2O 14.8% of the activity with N,N-dimethylformamide Pseudomonas sp. DMF 3/3 methylamine + formate
-
 ?
prolinamide + H2O 1.6% of the activity with N,N-dimethylformamide Pseudomonas sp. NH3 + proline
-
 ?

Subunits

Subunits Comment Organism
tetramer alpha2beta2, 2 * 15000 + 2 * 105000, SDS-PAGE Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Pseudomonas sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 80 20°C: 20% of activity maximum, 80°C: 10% of activity maximum Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20
-
 optimal stability below Pseudomonas sp.
60
-
 30 min, complete loss of activity Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 6
-
 Pseudomonas sp.
7.5
-
 optimal pH for temperatures of 45°C and 50°C Pseudomonas sp.

pH Range

pH Minimum pH Maximum Comment Organism
3 9.5 pH 3: 72% of activity maximum, pH 9.5: 73% of activity maximum Pseudomonas sp.