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Literature summary for 3.5.1.55 extracted from

  • Shintani, Y.; Fukuda, H.; Okamoto, N.; Murata, K.; Kimura, A.
    Isolation and characterization of N-long chain acyl aminoacylase from Pseudomonas diminuta (1984), J. Biochem., 96, 637-643.
    View publication on PubMed
Search for more literature for 3.5.1.55

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol
-
 Brevundimonas diminuta
DTT
-
 Brevundimonas diminuta
L-cysteine
-
 Brevundimonas diminuta
reduced glutathione
-
 Brevundimonas diminuta

Inhibitors

Inhibitors Comment Organism Structure
Hg2+ HgCl2 Brevundimonas diminuta
iodoacetamide
-
 Brevundimonas diminuta
PCMB
-
 Brevundimonas diminuta

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4
-
 N-lauroyl-L-aspartate
-
 Brevundimonas diminuta
2
-
 N-stearoyl-L-glutamate
-
 Brevundimonas diminuta
2
-
 N-lauroylglycine
-
 Brevundimonas diminuta
4.9
-
 N-palmitoyl-L-glutamate
-
 Brevundimonas diminuta
5
-
 N-myristoyl-L-glutamate
-
 Brevundimonas diminuta
7.1
-
 N-lauroyl-L-glutamate
-
 Brevundimonas diminuta

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 8 * 40000, enzyme I, SDS -PAGE Brevundimonas diminuta
55000
-
 4 * 55000, enzyme II, SDS-PAGE Brevundimonas diminuta
220000
-
 enzyme II, gel filtration Brevundimonas diminuta

Organism

Organism UniProt Comment Textmining
Brevundimonas diminuta
-
-
-

Purification (Commentary)

Purification (Comment) Organism
enzyme II Brevundimonas diminuta

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1857
-
-
 Brevundimonas diminuta

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme I is almost specific to the lipoamino acids containing L-glutamate Brevundimonas diminuta ?
-
 ?
additional information enzyme II shows broader substrate specificity than enzyme I and catalyzes the hydrolysis of lipoamino acids containing various amino acid residues Brevundimonas diminuta ?
-
 ?
N-lauroyl-L-aspartate + H2O 54% of the activity with palmitoyl-L-glutamate Brevundimonas diminuta lauroate + L-aspartate
-
 ?
N-lauroyl-L-glutamate + H2O 85% of the activity with palmitoyl-L-glutamate Brevundimonas diminuta lauroate + L-glutamate
-
 ?
N-lauroylglycine + H2O 17.5% of the activity with palmitoyl-L-glutamate Brevundimonas diminuta lauroate + glycine
-
 ?
N-myristoyl-L-glutamate + H2O 91% of the activity with palmitoyl-L-glutamate Brevundimonas diminuta myristoate + L-glutamate
-
 ?
N-myristoyl-L-glutamate + H2O 51% of the activity with N-decanoyl-L-glutamate Brevundimonas diminuta myristoate + L-glutamate
-
 ?
N-palmitoyl-L-glutamate + H2O best substrate Brevundimonas diminuta palmitate + L-glutamate
-
 ?
N-stearoyl-L-glutamate + H2O 28% of the activity with palmitoyl-L-glutamate Brevundimonas diminuta stearate + L-glutamate
-
 ?
N-undecanoyl-L-glutamate + H2O 51% of the activity with palmitoyl-L-glutamate Brevundimonas diminuta undecanoate + L-glutamate
-
 ?

Subunits

Subunits Comment Organism
octamer 8 * 40000, enzyme I, SDS -PAGE Brevundimonas diminuta
tetramer 4 * 55000, enzyme II, SDS-PAGE Brevundimonas diminuta

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Brevundimonas diminuta

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 pH 7.0, 10 min, 50% loss of activity, enzyme II Brevundimonas diminuta
55
-
 pH 7, 10 min, almost complete loss of activity, enzyme II Brevundimonas diminuta

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 9 substrate lauroyl-DL-valine, lauroylglycine Brevundimonas diminuta
8
-
 substrate N-lauroyl-L-glutamate, N-myristoyl-L-glutamate Brevundimonas diminuta
8
-
 N-lauroyl-L-aspartate, N-lauroyl-L-phenylalanine, N-palmitoyl-L-glutamate Brevundimonas diminuta

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 11 4°C, 24 h, stable, enzyme form II Brevundimonas diminuta