BRENDA - Enzyme Database
show all sequences of 3.5.1.54

Purification and properties of the allophanate hydrolase from Chlamydomonas reinhardii

Maitz, G.S.; Haas, E.M.; Castric, P.A.; Biochim. Biophys. Acta 714, 486-491 (1982)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
5,5'-dithiobis(2-nitrobenzoic acid)
-
Chlamydomonas reinhardtii
acetoacetic acid
Ki 0.3 mM
Chlamydomonas reinhardtii
malonic acid
-
Chlamydomonas reinhardtii
NEM
allophanate protects
Chlamydomonas reinhardtii
oxaloacetic acid
-
Chlamydomonas reinhardtii
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.55
-
allophanate
-
Chlamydomonas reinhardtii
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
100000
-
gel filtration
Chlamydomonas reinhardtii
110000
-
PAGE
Chlamydomonas reinhardtii
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
allophanate + H2O
Chlamydomonas reinhardtii
2nd step in the degradation of urea
CO2 + NH3
-
-
ir
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Chlamydomonas reinhardtii
-
-
-
Purification (Commentary)
Commentary
Organism
-
Chlamydomonas reinhardtii
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
19.3
-
-
Chlamydomonas reinhardtii
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
allophanate + H2O
-
209196
Chlamydomonas reinhardtii
CO2 + NH3
-
-
-
ir
allophanate + H2O
2nd step in the degradation of urea
209196
Chlamydomonas reinhardtii
CO2 + NH3
-
-
-
ir
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
-
Chlamydomonas reinhardtii
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7
10
pH 7.0: about 65% of maximal activity, pH 10.0: about 30% of maximal activity
Chlamydomonas reinhardtii
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.3
-
acetoacetic acid
-
Chlamydomonas reinhardtii
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
5,5'-dithiobis(2-nitrobenzoic acid)
-
Chlamydomonas reinhardtii
acetoacetic acid
Ki 0.3 mM
Chlamydomonas reinhardtii
malonic acid
-
Chlamydomonas reinhardtii
NEM
allophanate protects
Chlamydomonas reinhardtii
oxaloacetic acid
-
Chlamydomonas reinhardtii
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.3
-
acetoacetic acid
-
Chlamydomonas reinhardtii
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.55
-
allophanate
-
Chlamydomonas reinhardtii
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
100000
-
gel filtration
Chlamydomonas reinhardtii
110000
-
PAGE
Chlamydomonas reinhardtii
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
allophanate + H2O
Chlamydomonas reinhardtii
2nd step in the degradation of urea
CO2 + NH3
-
-
ir
Purification (Commentary) (protein specific)
Commentary
Organism
-
Chlamydomonas reinhardtii
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
19.3
-
-
Chlamydomonas reinhardtii
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
allophanate + H2O
-
209196
Chlamydomonas reinhardtii
CO2 + NH3
-
-
-
ir
allophanate + H2O
2nd step in the degradation of urea
209196
Chlamydomonas reinhardtii
CO2 + NH3
-
-
-
ir
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
-
Chlamydomonas reinhardtii
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7
10
pH 7.0: about 65% of maximal activity, pH 10.0: about 30% of maximal activity
Chlamydomonas reinhardtii
Other publictions for EC 3.5.1.54
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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Balotra
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470-480
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1
1
2
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1
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1
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1
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1
1
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1
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1
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2
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1
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1
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2
3
1
1
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1
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2
2
-
-
-
733034
Balotra
Crystallization and preliminar ...
Pseudomonas sp.
Acta crystallogr. Sect. F
70
310-315
2014
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-
1
1
1
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-
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2
1
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3
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1
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2
1
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1
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1
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2
1
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2
2
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733342
Lin
The structure of allophanate h ...
Granulibacter bethesdensis, Granulibacter bethesdensis ATCC BAA-1260
Biochemistry
52
690-700
2013
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-
1
1
8
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2
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1
2
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3
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1
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4
1
1
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1
1
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1
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1
8
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1
2
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1
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4
1
1
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1
1
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3
3
-
8
8
734220
Fan
Structure and function of allo ...
Kluyveromyces lactis
J. Biol. Chem.
288
21422-21432
2013
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1
1
3
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1
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2
2
-
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718475
Jacques
The structure of TTHA0988 from ...
no activity in Thermus thermophilus
Acta Crystallogr. Sect. D
67
105-111
2011
-
-
-
-
-
-
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3
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667267
Shapir
Purification and characterizat ...
Enterobacter cloacae, Enterobacter cloacae 99
Appl. Environ. Microbiol.
72
2491-2495
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-
-
1
-
-
-
1
1
-
1
3
2
-
6
-
-
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1
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10
1
1
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1
1
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1
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1
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1
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1
3
2
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1
-
10
1
1
-
-
1
1
-
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-
-
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-
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-
667259
Cheng
Allophanate hydrolase, not ure ...
Agrobacterium tumefaciens, Agrobacterium tumefaciens J14a, Enterobacter cloacae, Enterobacter cloacae 99, Herbaspirillum huttiense, Herbaspirillum huttiense NRRL B-12228, Pseudomonas sp., Ralstonia pickettii, Ralstonia pickettii D
Appl. Environ. Microbiol.
71
4437-4445
2005
-
-
4
-
-
-
-
-
-
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-
9
-
18
-
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19
-
1
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1
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4
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9
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19
-
1
-
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1
-
-
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-
-
-
-
668661
Kanamori
Allophanate hydrolase of Oleom ...
Oleomonas sagaranensis, Oleomonas sagaranensis HD-1
FEMS Microbiol. Lett.
245
61-65
2005
-
-
1
-
-
-
-
1
-
-
2
2
-
4
-
-
1
1
-
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1
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4
1
1
-
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1
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1
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1
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2
2
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1
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1
-
4
1
1
-
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-
1
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669098
Shapir
Purification and characterizat ...
Pseudomonas sp.
J. Bacteriol.
187
3731-3738
2005
-
-
1
-
3
-
1
2
-
-
2
1
-
4
-
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1
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1
-
6
1
-
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2
1
1
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1
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1
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3
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1
1
2
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2
1
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1
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1
-
6
1
-
-
-
2
1
1
-
-
-
-
-
-
-
-
209193
Nishiya
-
Production of urea amidolase b ...
Cyberlindnera jadinii, Cyberlindnera jadinii CA (u)-37
Seibutsu Shiro Bunseki
18
288-293
1995
-
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2
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1
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-
1609
Sumrada
Urea carboxylase and allophana ...
Saccharomyces cerevisiae
J. Biol. Chem.
257
9119-9127
1982
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-
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1
1
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209196
Maitz
-
Purification and properties of ...
Chlamydomonas reinhardtii
Biochim. Biophys. Acta
714
486-491
1982
-
-
-
-
-
-
5
1
-
-
2
1
-
1
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1
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1
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2
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1
1
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1
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5
1
1
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2
1
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1
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1
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2
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1
1
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1602
Whitney
Urea carboxylase and allophana ...
Saccharomyces cerevisiae
Biochem. Biophys. Res. Commun.
49
45-51
1972
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1
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1
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