BRENDA - Enzyme Database show
show all sequences of 3.5.1.53

Occurrence of agmatine pathway for putrescine synthesis in Selenomonas ruminatium

Liao, S.; Poonpairoj, P.; Ko, K.; Takatuska, Y.; Yamaguchi, Y.; Abe, N.; Kaneko, J.; Kamio, Y.; Biosci. Biotechnol. Biochem. 72, 445-455 (2008)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli; expression in Escherichia coli strain DE3
Selenomonas ruminantium
Engineering
Amino acid exchange
Commentary
Organism
C153A
inactive mutant enzyme; mutant without enzyme activity
Selenomonas ruminantium
E160A
inactive mutant enzyme; mutant without enzyme activity
Selenomonas ruminantium
General Stability
General Stability
Organism
in 50 mM PIPES buffer at pH 6.5 stable for 30 min up to 70°C
Selenomonas ruminantium
Inhibitors
Inhibitors
Commentary
Organism
Structure
Co2+
; strong inhibitor
Selenomonas ruminantium
Fe2+
; strong inhibitor
Selenomonas ruminantium
Hg2+
; strong inhibitor
Selenomonas ruminantium
iodoacetamide
; strong inhibitor
Selenomonas ruminantium
Mn2+
; strong inhibitor
Selenomonas ruminantium
Zn2+
; strong inhibitor
Selenomonas ruminantium
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.22
-
N-Carbamoylputrescine
;
Selenomonas ruminantium
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32532
-
2 * 32532, calculated from sequence
Selenomonas ruminantium
37000
-
2 * 37000, SDS-PAGE; SDS-PAGE
Selenomonas ruminantium
80000
-
gel filtration; gel filtration
Selenomonas ruminantium
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Selenomonas ruminantium
Q5KR04
-
-
Selenomonas ruminantium
-
-
-
Purification (Commentary)
Commentary
Organism
; recombinant
Selenomonas ruminantium
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
N-carbamoylputrescine + H2O
-
685687
Selenomonas ruminantium
putrescine + CO2 + NH3
-
-
-
?
N-carbamoylputrescine + H2O
10 mM, assay at pH 6.5, 10 min, 40°C
685687
Selenomonas ruminantium
putrescine + CO2 + NH3
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 32532, calculated from sequence; 2 * 37000, SDS-PAGE
Selenomonas ruminantium
homodimer
-
Selenomonas ruminantium
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
45
-
; assay at pH 6.5, 10 min, 40°C
Selenomonas ruminantium
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
70
-
AguB activity is stable at up to 70°C for 30 min in 50 mM PIPES buffer, pH 6.5; up to 70°C, stable for 30 min at pH 6.5, 50 mM PIPES buffer
Selenomonas ruminantium
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
; assay at pH 6.5, 10 min, 40°C
Selenomonas ruminantium
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
6
8
; stable
Selenomonas ruminantium
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli
Selenomonas ruminantium
expression in Escherichia coli strain DE3
Selenomonas ruminantium
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
C153A
inactive mutant enzyme
Selenomonas ruminantium
C153A
mutant without enzyme activity
Selenomonas ruminantium
E160A
inactive mutant enzyme
Selenomonas ruminantium
E160A
mutant without enzyme activity
Selenomonas ruminantium
General Stability (protein specific)
General Stability
Organism
in 50 mM PIPES buffer at pH 6.5 stable for 30 min up to 70°C
Selenomonas ruminantium
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Co2+
-
Selenomonas ruminantium
Co2+
strong inhibitor
Selenomonas ruminantium
Fe2+
-
Selenomonas ruminantium
Fe2+
strong inhibitor
Selenomonas ruminantium
Hg2+
-
Selenomonas ruminantium
Hg2+
strong inhibitor
Selenomonas ruminantium
iodoacetamide
-
Selenomonas ruminantium
iodoacetamide
strong inhibitor
Selenomonas ruminantium
Mn2+
-
Selenomonas ruminantium
Mn2+
strong inhibitor
Selenomonas ruminantium
Zn2+
-
Selenomonas ruminantium
Zn2+
strong inhibitor
Selenomonas ruminantium
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.22
-
N-Carbamoylputrescine
-
Selenomonas ruminantium
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32532
-
2 * 32532, calculated from sequence
Selenomonas ruminantium
37000
-
2 * 37000, SDS-PAGE
Selenomonas ruminantium
37000
-
SDS-PAGE
Selenomonas ruminantium
80000
-
gel filtration
Selenomonas ruminantium
Purification (Commentary) (protein specific)
Commentary
Organism
-
Selenomonas ruminantium
recombinant
Selenomonas ruminantium
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
N-carbamoylputrescine + H2O
-
685687
Selenomonas ruminantium
putrescine + CO2 + NH3
-
-
-
?
N-carbamoylputrescine + H2O
10 mM, assay at pH 6.5, 10 min, 40°C
685687
Selenomonas ruminantium
putrescine + CO2 + NH3
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 32532, calculated from sequence; 2 * 37000, SDS-PAGE
Selenomonas ruminantium
homodimer
-
Selenomonas ruminantium
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
45
-
-
Selenomonas ruminantium
45
-
assay at pH 6.5, 10 min, 40°C
Selenomonas ruminantium
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
70
-
AguB activity is stable at up to 70°C for 30 min in 50 mM PIPES buffer, pH 6.5
Selenomonas ruminantium
70
-
up to 70°C, stable for 30 min at pH 6.5, 50 mM PIPES buffer
Selenomonas ruminantium
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Selenomonas ruminantium
7
-
assay at pH 6.5, 10 min, 40°C
Selenomonas ruminantium
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
6
8
stable
Selenomonas ruminantium
6
8
-
Selenomonas ruminantium
Other publictions for EC 3.5.1.53
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
713298
Fuell
Polyamine biosynthetic diversi ...
Arabidopsis thaliana
Plant Physiol. Biochem.
48
513-520
2010
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-
-
-
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1
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-
-
-
-
7
-
-
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1
1
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1
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1
1
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1
1
-
-
-
719620
Landete
The role of two families of ba ...
Pseudomonas aeruginosa
Int. Microbiol.
13
169-177
2010
-
-
-
-
-
-
-
-
-
-
-
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-
8
-
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1
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-
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-
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-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
685687
Liao
Occurrence of agmatine pathway ...
Selenomonas ruminantium
Biosci. Biotechnol. Biochem.
72
445-455
2008
-
-
1
-
2
1
6
1
-
-
3
-
-
6
-
-
1
-
-
-
-
-
2
2
1
-
1
-
1
-
1
-
-
-
-
-
-
2
-
-
4
1
-
12
-
2
-
-
5
-
-
-
-
2
-
-
-
-
2
2
2
-
2
-
2
-
2
-
-
-
-
-
-
-
692846
Chou
Transcriptome analysis of agma ...
Pseudomonas aeruginosa
J. Bacteriol.
190
1966-1975
2008
1
-
1
-
-
-
-
-
-
2
-
2
-
1
-
-
-
-
-
-
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
690188
Baumann
Chlorella viruses contain gene ...
Paramecium bursaria Chlorella virus 1, Paramecium bursaria chlorella virus MT325, Paramecium bursaria Chlorella virus NY2A
Virology
360
209-217
2007
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-
3
-
-
-
-
-
-
-
-
-
-
9
-
-
3
-
-
-
-
-
3
-
-
-
-
-
-
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-
-
-
-
-
-
-
3
-
-
-
-
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3
-
-
-
-
3
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
652383
Piotrowski
Plant C-N hydrolases and the i ...
Arabidopsis thaliana
J. Biol. Chem.
278
1708-1712
2003
-
-
1
-
-
-
-
1
-
-
1
-
-
6
-
-
1
-
-
1
-
-
1
-
1
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
1
-
1
-
-
1
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
653166
Nakada
Identification of the putresci ...
Pseudomonas aeruginosa
Microbiology
149
707-714
2003
-
-
-
-
-
-
4
1
-
-
2
-
-
3
-
-
-
-
-
-
-
-
3
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
1
-
-
2
-
-
-
-
-
-
-
-
-
3
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
209192
Mendum
-
Substrate utilization by grape ...
Vitis vinifera
Am. J. Enol. Vitic.
48
137-144
1997
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
10
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
10
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
209191
Shoeb
-
Diguanidinobutane (arcaine) de ...
Rhodococcus sp., Rhodococcus sp. C-x
Biosci. Biotechnol. Biochem.
58
859-863
1994
-
-
-
-
-
-
-
-
-
-
-
2
-
3
-
-
1
-
-
-
3
-
8
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
3
-
8
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
209189
Yanagisawa
-
Purification and properties of ...
Zea mays
Phytochemistry
21
2201-2203
1982
-
-
-
-
-
-
3
1
1
-
1
1
-
1
-
-
1
-
-
1
-
1
2
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
1
1
-
1
1
-
-
-
1
-
1
-
1
2
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
209190
Mercenier
Catabolism of L-arginine by Ps ...
Pseudomonas aeruginosa
J. Gen. Microbiol.
116
381-389
1980
-
-
-
-
-
-
-
1
-
-
-
1
-
2
-
-
-
-
-
-
-
-
2
-
-
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-
1
-
-
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1
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1
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2
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-
1
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-
-
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