BRENDA - Enzyme Database
show all sequences of 3.5.1.53

Identification of the putrescine biosynthetic genes in Pseudomonas aeruginosa and characterization of agmatine deiminase and N-carbamoylputrescine amidohydrolase of the arginine decarboxylase pathway

Nakada, Y.; Itoh, Y.; Microbiology 149, 707-714 (2003)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Hg2+
79% inhibition at 0.1 mM
Pseudomonas aeruginosa
iodoacetamide
88% inhibition at 1 mM
Pseudomonas aeruginosa
additional information
not inhibitory at 1 mM: arginine, ornithine, EDTA, putrescine, spermidine, spermine
Pseudomonas aeruginosa
p-chloromercuribenzoate
94% inhibition at 0.1 mM
Pseudomonas aeruginosa
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.5
-
N-Carbamoylputrescine
-
Pseudomonas aeruginosa
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
33000
-
6 * 33000, SDS-PAGE
Pseudomonas aeruginosa
230000
-
gel filtration
Pseudomonas aeruginosa
Organism
Organism
UniProt
Commentary
Textmining
Pseudomonas aeruginosa
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
N-carbamoylcadaverine + H2O
poor substrate
653166
Pseudomonas aeruginosa
cadaverine + CO2 + NH3
-
653166
Pseudomonas aeruginosa
?
N-carbamoyldiaminopropane + H2O
poor substrate
653166
Pseudomonas aeruginosa
diaminopropane + CO2 + NH3
-
653166
Pseudomonas aeruginosa
?
N-carbamoylputrescine + H2O
-
653166
Pseudomonas aeruginosa
putrescine + CO2 + NH3
-
653166
Pseudomonas aeruginosa
?
Subunits
Subunits
Commentary
Organism
homohexamer
6 * 33000, SDS-PAGE
Pseudomonas aeruginosa
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
40
-
-
Pseudomonas aeruginosa
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Pseudomonas aeruginosa
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Hg2+
79% inhibition at 0.1 mM
Pseudomonas aeruginosa
iodoacetamide
88% inhibition at 1 mM
Pseudomonas aeruginosa
additional information
not inhibitory at 1 mM: arginine, ornithine, EDTA, putrescine, spermidine, spermine
Pseudomonas aeruginosa
p-chloromercuribenzoate
94% inhibition at 0.1 mM
Pseudomonas aeruginosa
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.5
-
N-Carbamoylputrescine
-
Pseudomonas aeruginosa
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
33000
-
6 * 33000, SDS-PAGE
Pseudomonas aeruginosa
230000
-
gel filtration
Pseudomonas aeruginosa
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
N-carbamoylcadaverine + H2O
poor substrate
653166
Pseudomonas aeruginosa
cadaverine + CO2 + NH3
-
653166
Pseudomonas aeruginosa
?
N-carbamoyldiaminopropane + H2O
poor substrate
653166
Pseudomonas aeruginosa
diaminopropane + CO2 + NH3
-
653166
Pseudomonas aeruginosa
?
N-carbamoylputrescine + H2O
-
653166
Pseudomonas aeruginosa
putrescine + CO2 + NH3
-
653166
Pseudomonas aeruginosa
?
Subunits (protein specific)
Subunits
Commentary
Organism
homohexamer
6 * 33000, SDS-PAGE
Pseudomonas aeruginosa
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
40
-
-
Pseudomonas aeruginosa
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Pseudomonas aeruginosa
Other publictions for EC 3.5.1.53
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
713298
Fuell
Polyamine biosynthetic diversi ...
Arabidopsis thaliana
Plant Physiol. Biochem.
48
513-520
2010
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1
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1
1
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719620
Landete
The role of two families of ba ...
Pseudomonas aeruginosa
Int. Microbiol.
13
169-177
2010
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8
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1
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685687
Liao
Occurrence of agmatine pathway ...
Selenomonas ruminantium
Biosci. Biotechnol. Biochem.
72
445-455
2008
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-
1
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2
1
6
1
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3
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6
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5
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4
1
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12
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2
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5
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2
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2
2
2
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2
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2
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2
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692846
Chou
Transcriptome analysis of agma ...
Pseudomonas aeruginosa
J. Bacteriol.
190
1966-1975
2008
1
-
1
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2
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2
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1
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4
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1
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-
-
1
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690188
Baumann
Chlorella viruses contain gene ...
Paramecium bursaria Chlorella virus 1, Paramecium bursaria chlorella virus MT325, Paramecium bursaria Chlorella virus NY2A
Virology
360
209-217
2007
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3
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9
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3
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3
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3
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652383
Piotrowski
Plant C-N hydrolases and the i ...
Arabidopsis thaliana
J. Biol. Chem.
278
1708-1712
2003
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1
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1
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6
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1
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653166
Nakada
Identification of the putresci ...
Pseudomonas aeruginosa
Microbiology
149
707-714
2003
-
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4
1
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2
-
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3
-
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3
1
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1
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1
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4
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1
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3
1
1
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1
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209192
Mendum
-
Substrate utilization by grape ...
Vitis vinifera
Am. J. Enol. Vitic.
48
137-144
1997
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1
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1
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1
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10
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1
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10
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1
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209191
Shoeb
-
Diguanidinobutane (arcaine) de ...
Rhodococcus sp., Rhodococcus sp. C-x
Biosci. Biotechnol. Biochem.
58
859-863
1994
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8
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209189
Yanagisawa
-
Purification and properties of ...
Zea mays
Phytochemistry
21
2201-2203
1982
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3
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1
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1
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209190
Mercenier
Catabolism of L-arginine by Ps ...
Pseudomonas aeruginosa
J. Gen. Microbiol.
116
381-389
1980
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