BRENDA - Enzyme Database
show all sequences of 3.5.1.53

Plant C-N hydrolases and the identification of a plant N-carbamoylputrescine amidohydrolase involved in polyamine biosynthesis

Piotrowski, M.; Janowitz, T.; Kneifel, H.; J. Biol. Chem. 278, 1708-1712 (2003)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli, His6-tag
Arabidopsis thaliana
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.135
-
N-Carbamoylputrescine
pH 8.0, 30°C
Arabidopsis thaliana
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
279000
-
PAGE
Arabidopsis thaliana
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
-
-
-
Purification (Commentary)
Commentary
Organism
recombinant protein
Arabidopsis thaliana
Source Tissue
Source Tissue
Commentary
Organism
Textmining
additional information
found in all organs investigated
Arabidopsis thaliana
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
N-carbamoylputrescine + H2O
-
652383
Arabidopsis thaliana
putrescine + CO2 + NH3
-
652383
Arabidopsis thaliana
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
-
Arabidopsis thaliana
pH Range
pH Minimum
pH Maximum
Commentary
Organism
8
9
-
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli, His6-tag
Arabidopsis thaliana
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.135
-
N-Carbamoylputrescine
pH 8.0, 30°C
Arabidopsis thaliana
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
279000
-
PAGE
Arabidopsis thaliana
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant protein
Arabidopsis thaliana
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
additional information
found in all organs investigated
Arabidopsis thaliana
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
N-carbamoylputrescine + H2O
-
652383
Arabidopsis thaliana
putrescine + CO2 + NH3
-
652383
Arabidopsis thaliana
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
-
Arabidopsis thaliana
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
8
9
-
Arabidopsis thaliana
Other publictions for EC 3.5.1.53
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
713298
Fuell
Polyamine biosynthetic diversi ...
Arabidopsis thaliana
Plant Physiol. Biochem.
48
513-520
2010
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1
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7
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1
1
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1
1
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1
1
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719620
Landete
The role of two families of ba ...
Pseudomonas aeruginosa
Int. Microbiol.
13
169-177
2010
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8
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1
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685687
Liao
Occurrence of agmatine pathway ...
Selenomonas ruminantium
Biosci. Biotechnol. Biochem.
72
445-455
2008
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1
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2
1
6
1
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3
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6
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1
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2
2
1
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1
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1
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1
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2
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4
1
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12
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2
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5
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2
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2
2
2
-
2
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2
-
2
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692846
Chou
Transcriptome analysis of agma ...
Pseudomonas aeruginosa
J. Bacteriol.
190
1966-1975
2008
1
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1
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2
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2
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1
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4
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1
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1
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1
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2
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2
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4
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1
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1
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690188
Baumann
Chlorella viruses contain gene ...
Paramecium bursaria Chlorella virus 1, Paramecium bursaria chlorella virus MT325, Paramecium bursaria Chlorella virus NY2A
Virology
360
209-217
2007
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3
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9
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3
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3
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3
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3
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3
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652383
Piotrowski
Plant C-N hydrolases and the i ...
Arabidopsis thaliana
J. Biol. Chem.
278
1708-1712
2003
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1
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1
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1
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6
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1
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1
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1
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1
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1
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1
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1
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653166
Nakada
Identification of the putresci ...
Pseudomonas aeruginosa
Microbiology
149
707-714
2003
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4
1
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2
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3
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3
1
1
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1
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4
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1
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3
1
1
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1
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209192
Mendum
-
Substrate utilization by grape ...
Vitis vinifera
Am. J. Enol. Vitic.
48
137-144
1997
-
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1
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1
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1
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10
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1
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1
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1
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10
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1
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209191
Shoeb
-
Diguanidinobutane (arcaine) de ...
Rhodococcus sp., Rhodococcus sp. C-x
Biosci. Biotechnol. Biochem.
58
859-863
1994
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1
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3
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8
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1
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209189
Yanagisawa
-
Purification and properties of ...
Zea mays
Phytochemistry
21
2201-2203
1982
-
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3
1
1
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1
1
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1
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1
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1
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1
2
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1
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1
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1
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2
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1
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1
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209190
Mercenier
Catabolism of L-arginine by Ps ...
Pseudomonas aeruginosa
J. Gen. Microbiol.
116
381-389
1980
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1
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1
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