Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 cells | Neurospora crassa |
Protein Variants | Comment | Organism |
---|---|---|
A208C | inactive | Neurospora crassa |
A208C/Y235H | inactive | Neurospora crassa |
C221T | mutant with more than 50% wild type activity | Saccharomyces cerevisiae |
D179E/P180A | mutant with more than 50% wild type activity | Saccharomyces cerevisiae |
D208R/V209A | mutant with 10-50% wild type activity | Saccharomyces cerevisiae |
E185R | mutant with 10-50% wild type activity | Saccharomyces cerevisiae |
E185R/T186V | mutant with 10-50% wild type activity | Saccharomyces cerevisiae |
E193D | inactive | Saccharomyces cerevisiae |
E193D/W194F | inactive | Saccharomyces cerevisiae |
F224Y | mutant with more than 50% wild type activity | Saccharomyces cerevisiae |
G206D/L207I | mutant with 10-50% wild type activity | Saccharomyces cerevisiae |
I181W | mutant with more than 50% wild type activity | Saccharomyces cerevisiae |
I181W/K182Q | inactive | Saccharomyces cerevisiae |
K182Q | mutant with more than 50% wild type activity | Saccharomyces cerevisiae |
L198V | mutant with more than 50% wild type activity | Saccharomyces cerevisiae |
L200M | mutant with more than 50% wild type activity | Saccharomyces cerevisiae |
L200M/I201L | mutant with more than 50% wild type activity | Saccharomyces cerevisiae |
N178T | mutant with 10-50% wild type activity | Saccharomyces cerevisiae |
N214C/R215Q | mutant with 10-50% wild type activity | Saccharomyces cerevisiae |
N266F/F227H | mutant with more than 50% wild type activity | Saccharomyces cerevisiae |
R187K/K188R | mutant with more than 50% wild type activity | Saccharomyces cerevisiae |
V219L | mutant with 1-10% wild type activity | Saccharomyces cerevisiae |
W194F/C195A | mutant with 10-50% wild type activity | Saccharomyces cerevisiae |
Y211W | mutant with more than 50% wild type activity | Saccharomyces cerevisiae |
Y223I | mutant with 10-50% wild type activity | Saccharomyces cerevisiae |
Y235H | inactive | Neurospora crassa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neurospora crassa | - |
- |
- |
Saccharomyces cerevisiae | - |
- |
- |
Synonyms | Comment | Organism |
---|---|---|
peptide:N-glycanase | - |
Saccharomyces cerevisiae |
peptide:N-glycanase | - |
Neurospora crassa |
PNG1 | - |
Saccharomyces cerevisiae |
PNG1 | wild type PNG1 has substitutions in essential catalytic amino acids, and its deglycosylation activity is lost | Neurospora crassa |
PNGase | - |
Saccharomyces cerevisiae |
PNGase | - |
Neurospora crassa |
General Information | Comment | Organism |
---|---|---|
malfunction | PNG1 deletion results in strong polarity defects | Neurospora crassa |
physiological function | the peptide:N-glycanase ortholog PNG1 is essential for cell polarity despite its lack of enzymatic activity, PNG1 is involved in cell wall integrity | Neurospora crassa |