Literature summary for 3.5.1.52 extracted from

Su, Y.; Zhang, H.; Gu, L.; Chen, M.; Xiao, M.; Wang, P.G.; Qi, Q.
Engineered yeast with PNGase F on cell surface for releasing of N-glycans from glycoproteins (2007), Enzyme Microb. Technol., 40, 1496-1502.

No PubMed abstract available

Cloned(Commentary)

Cloned (Comment)	Organism
the PNGase F gene from Flavobacterium meningosepticum is cloned into plasmid pYD1, which enables regulated expression, secretion and detection. The expression of PNGase F gene at extracellular surface of Saccharomyces cerevisiae is confirmed by immunofluorescence microscopy. Fluorescence activated cell sorter analysis indicates that, after 36 h cultivation, 47.6% of the cell surface is anchored with target proteins. The surface engineered enzyme is confirmed to be active and reached its highest level after induced for 36 h	Elizabethkingia meningoseptica

Cloned (Comment)

Organism

the PNGase F gene from Flavobacterium meningosepticum is cloned into plasmid pYD1, which enables regulated expression, secretion and detection. The expression of PNGase F gene at extracellular surface of Saccharomyces cerevisiae is confirmed by immunofluorescence microscopy. Fluorescence activated cell sorter analysis indicates that, after 36 h cultivation, 47.6% of the cell surface is anchored with target proteins. The surface engineered enzyme is confirmed to be active and reached its highest level after induced for 36 h

Elizabethkingia meningoseptica

Organism

Organism	UniProt	Comment	Textmining
Elizabethkingia meningoseptica	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
RNase B + H2O	-	Elizabethkingia meningoseptica	?	-	?

Synonyms

Synonyms	Comment	Organism
peptide-N-(N-acetyl-beta-glucosaminyl) asparagine amidase F	-	Elizabethkingia meningoseptica
PNGase F	-	Elizabethkingia meningoseptica

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	surface-displayed PNGase F	Elizabethkingia meningoseptica

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
20	45	20°C: about 50% of maximal activity, 45°C: about 60% of maximal activity, surface-displayed PNGase F	Elizabethkingia meningoseptica

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5.5	-	surface-displayed PNGase F	Elizabethkingia meningoseptica

pH Range

pH Minimum	pH Maximum	Comment	Organism
4	7.5	pH 4.0: about 50% of maximal activity, pH 7.5: about 40% of maximal activity, surface-displayed PNGase F	Elizabethkingia meningoseptica