Any feedback?
Literature summary for 3.5.1.52 extracted from
- Molecular identification and characterization of peptide: N-glycanase from Schizosaccharomyces pombe (2008), Biochem. Biophys. Res. Commun., 368, 907-912.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Schizosaccharomyces pombe |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | not restored by readdition of metal ions | Schizosaccharomyces pombe |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | zinc metalloenzyme | Schizosaccharomyces pombe | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Schizosaccharomyces pombe | SpPNGase deglycosylates the misfolded glycoproteins | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Schizosaccharomyces pombe | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| denatured RNase B + H2O | SpPNGase cleaves N-glycan from denatured RNase B | Schizosaccharomyces pombe | ? | - |
? | |
| additional information | SpPNGase deglycosylates the misfolded glycoproteins | Schizosaccharomyces pombe | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| peptide: N-glycanase | - |
Schizosaccharomyces pombe |
| SpPNGase | - |
Schizosaccharomyces pombe |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Schizosaccharomyces pombe |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 40 | 20°C: about 80% of maximal activity, 40°C: about 75% of maximal activity | Schizosaccharomyces pombe |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Schizosaccharomyces pombe |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | pH 6: about 70% of maximal activity, pH 9.0: about 60% of maximal activity | Schizosaccharomyces pombe |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
