Cloned (Comment) | Organism |
---|---|
gene png1, expression of wild-type and mutant Png1 in Escherichia coli strain BL21(DE3) | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
C191A | site-directed mutagenesis, inactive mutant | Saccharomyces cerevisiae |
D235A | site-directed mutagenesis, inactive mutant | Saccharomyces cerevisiae |
H218A | site-directed mutagenesis, inactive mutant | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant Png1 from Escherichia coli strain BL21(DE3) | Saccharomyces cerevisiae |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
Manalpha(1-6)(Xylbeta(1-2))Manbeta(1-4)GlcNAcbeta(1-4)(Fucalpha(1-3))GlcNAc-Asn-Asp-Glu-Ser-Ser + H2O = Manalpha(1-6)(Xylbeta(1-2))Manbeta(1-4)GlcNAcbeta(1-4)(Fucalpha(1-3))GlcNAc + Asn-Asp-Glu-Ser-Ser | the catalytic triad is formed by Cys191, His218, and Asp235 | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the deglycosylating enzyme catalyzes the hydrolysis of the beta-aspartylglycosylamine bond of asparagine-linked glycopeptides and glycoproteins, the misfolding or denaturation of glycoproteins is a prerequisite for enzyme activity | Saccharomyces cerevisiae | ? | - |
? | |
ovalbumin + H2O | deglycosylation of the heat-denatured protein | Saccharomyces cerevisiae | ? | - |
? | |
yeast carboxypeptidase + H2O | deglycosylation of heat-denatured protein | Saccharomyces cerevisiae | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
peptide: N-glycanase | - |
Saccharomyces cerevisiae |
Png1p | - |
Saccharomyces cerevisiae |
PNGase | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Saccharomyces cerevisiae |