Literature summary for 3.5.1.52 extracted from

Katiyar, S.; Suzuki, T.; Balgobin, B.J.; Lennarz, W.J.
Site-directed mutagenesis study of yeast peptide: N-glycanase. Insight into the reaction mechanism of deglycosylation (2002), J. Biol. Chem., 277, 12953-12959.

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Protein Variants

Protein Variants	Comment	Organism
C191A	no enzymic activity	Saccharomyces cerevisiae
D235A	no enzymic activity	Saccharomyces cerevisiae
E222A	no enzymic activity	Saccharomyces cerevisiae
H218A	no enzymic activity	Saccharomyces cerevisiae
additional information	site-directed mutagenesis of not conserved amino acids	Saccharomyces cerevisiae
R210A	no enzymic activity	Saccharomyces cerevisiae
W220F	81% of activity compared to wild type	Saccharomyces cerevisiae
W231F	83% of activity compared to wild type	Saccharomyces cerevisiae

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
Manalpha(1-6)(Xylbeta(1-2))Manbeta(1-4)GlcNAcbeta(1-4)(Fucalpha(1-3))GlcNAc-Asn-Asp-Glu-Ser-Ser + H2O = Manalpha(1-6)(Xylbeta(1-2))Manbeta(1-4)GlcNAcbeta(1-4)(Fucalpha(1-3))GlcNAc + Asn-Asp-Glu-Ser-Ser	mechanism and catalytic center	Saccharomyces cerevisiae	a

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Release 2023.1 (January 2023)