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Literature summary for 3.5.1.5 extracted from

  • Liu, X.; Zhang, Q.; Zhou, N.; Tian, Y.
    Expression of an acid urease with urethanase activity in E. coli and analysis of urease gene (2017), Mol. Biotechnol., 59, 84-97 .
    View publication on PubMed
Search for more literature for 3.5.1.5

Cloned(Commentary)

Cloned (Comment) Organism
gene ureC, functional recombinant expression of His-tagged enzyme from operon ureABC in Escherichia coli strain BL21(DE3) in inclusion bodies Providencia rettgeri

Inhibitors

Inhibitors Comment Organism Structure
ethanol 50% inhibition at 30% v/v Providencia rettgeri
Ni2+ Ni2+ is essential to urease activation, best at 0.5 mM. Urease is inhibited at high concentration of Ni2+ Providencia rettgeri

Metals/Ions

Metals/Ions Comment Organism Structure
Ni2+ Ni2+ is essential to urease activation, best at 0.5 mM. Urease is inhibited at high concentration of Ni2+ Providencia rettgeri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Providencia rettgeri the enzyme exhibits not only urease activity, but also urethanase (EC 3.5.1.75) activity ?
-
 ?
additional information Providencia rettgeri JN-B815 the enzyme exhibits not only urease activity, but also urethanase (EC 3.5.1.75) activity ?
-
 ?
additional information Providencia rettgeri CGMCC 8326 the enzyme exhibits not only urease activity, but also urethanase (EC 3.5.1.75) activity ?
-
 ?
urea + H2O Providencia rettgeri
-
 CO2 + 2 NH3
-
 ?
urea + H2O Providencia rettgeri JN-B815
-
 CO2 + 2 NH3
-
 ?
urea + H2O Providencia rettgeri CGMCC 8326
-
 CO2 + 2 NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Providencia rettgeri A0A1U8X5K7
-
-
Providencia rettgeri CGMCC 8326 A0A1U8X5K7
-
-
Providencia rettgeri JN-B815 A0A1U8X5K7
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme, refolded from Escherichia coli strain BL21(DE3) inclusion bodies, 1.75fold by nickel affinity chromatography Providencia rettgeri

Renatured (Commentary)

Renatured (Comment) Organism
recombinant His-tagged enzyme is solubilized and refolded from Escherichia coli strain BL21(DE3) inclusion bodies. Secondary structure analysis of renatured recombinant urease Providencia rettgeri

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10.7
-
 purified recombinant apoenzyme, urease activity, pH 4.5, temperature not specified in the publication Providencia rettgeri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme exhibits not only urease activity, but also urethanase (EC 3.5.1.75) activity Providencia rettgeri ?
-
 ?
additional information the enzyme exhibits not only urease activity, but also urethanase (EC 3.5.1.75) activity Providencia rettgeri JN-B815 ?
-
 ?
additional information the enzyme exhibits not only urease activity, but also urethanase (EC 3.5.1.75) activity Providencia rettgeri CGMCC 8326 ?
-
 ?
urea + H2O
-
 Providencia rettgeri CO2 + 2 NH3
-
 ?
urea + H2O
-
 Providencia rettgeri JN-B815 CO2 + 2 NH3
-
 ?
urea + H2O
-
 Providencia rettgeri CGMCC 8326 CO2 + 2 NH3
-
 ?

Synonyms

Synonyms Comment Organism
acid urease
-
 Providencia rettgeri
More also see for EC 3.5.1.75 Providencia rettgeri
UreC
-
 Providencia rettgeri

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Providencia rettgeri

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
 recombinant enzyme Providencia rettgeri

pH Range

pH Minimum pH Maximum Comment Organism
3 8.5 activity range, recombinant enzyme, profile overview Providencia rettgeri