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Literature summary for 3.5.1.5 extracted from

  • Rajendran, R.; Pandi, A.; Ramchary, A.; Thiagarajan, H.; Panneerselvam, J.; Niraikulam, A.; Kuppuswami, G.M.; Ramudu, K.N.
    Extracellular urease from Arthrobacter creatinolyticus MTCC 5604 scale up, purification and its cytotoxic effect thereof (2019), Mol. Biol. Rep., 46, 133-141 .
    View publication on PubMed

Application

Application Comment Organism
medicine site targeted delivery of urease along with chemotherapeutic drugs can be a promising tool for anti-cancer applications. Urease shows its inhibitory effects on cancer cell lines through the generation of toxic ammonia, which in turn increases the pH of the surrounding medium. This increase in extracellular pH, enhances the cytotoxic effect of weak base chemotherapeutic drugs, doxorubicin (0.05 mM) and vinblastine (0.100 mM) in the presence of urease (5 U/ml) and urea (0-4 mM) significantly Glutamicibacter creatinolyticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
16.21
-
Urea pH and temperature not specified in the publication Glutamicibacter creatinolyticus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Glutamicibacter creatinolyticus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
270000
-
non-denaturing PAGE Glutamicibacter creatinolyticus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
urea + H2O Glutamicibacter creatinolyticus
-
CO2 + 2 NH3
-
?
urea + H2O Glutamicibacter creatinolyticus MTCC 5604
-
CO2 + 2 NH3
-
?

Organism

Organism UniProt Comment Textmining
Glutamicibacter creatinolyticus
-
-
-
Glutamicibacter creatinolyticus MTCC 5604
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Glutamicibacter creatinolyticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
urea + H2O
-
Glutamicibacter creatinolyticus CO2 + 2 NH3
-
?
urea + H2O
-
Glutamicibacter creatinolyticus MTCC 5604 CO2 + 2 NH3
-
?

Subunits

Subunits Comment Organism
? the purified urease shows three bands with molecular weight of 72600 Da, 11200 Da and 6100 Da, SDS PAGE Glutamicibacter creatinolyticus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Glutamicibacter creatinolyticus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 80 20°C: about 40% of maximal activity, 80°C: about 45% of maximal activity Glutamicibacter creatinolyticus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4 50 1 h pH 8.0, stable between 4°C and 50°C Glutamicibacter creatinolyticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Glutamicibacter creatinolyticus

pH Range

pH Minimum pH Maximum Comment Organism
6 10 pH 6.0: about 40% of maximal activity, pH 10.0: about 50% of maximal activity Glutamicibacter creatinolyticus

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 9 1 h 30°C, stable within the pH range of 7.0-9.0 Glutamicibacter creatinolyticus