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Literature summary for 3.5.1.5 extracted from
- Synthesis and characterisation of biocompatible polymer-conjugated magnetic beads for enhancement stability of urease (2016), Appl. Biochem. Biotechnol., 179, 94-110 .
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | urease is used to calculate the amount of urea in biological solutions in order to remove urea from blood, waste water, fruit juice and foods. Enhancement of stability of immobilised urease by biocompatible polymer-conjugated magnetic beads for industrial application based on removal of urea | Canavalia ensiformis |
| industry | enhancement of stability of immobilised urease by biocompatible polymer-conjugated magnetic beads for industrial application based on removal of urea | Canavalia ensiformis |
General Stability
| General Stability | Organism |
|---|---|
| enhancement of stability of immobilised urease by biocompatible polymer-conjugated magnetic beads for the industrial application based on removal of urea significant increase in pH stability for urease-encapsulated magnetic beads compared with the free urease for both acidic and alkaline medium | Canavalia ensiformis |
| urease encapsulated in chitosan nickel ferrite beads shows 50% loss of activity after 18 reuses | Canavalia ensiformis |
| urease encapsulated in chitosan-coated alginate-magnetite shows 50% loss of activity after 14 reuses | Canavalia ensiformis |
| urease encapsulated in chitosan-coated alginate-nickel ferrite beads shows 50% loss of activity after 14 reuses | Canavalia ensiformis |
| urease encapsulated in chitosan-magnetite beads shows 50% loss of activity after 18 reuses | Canavalia ensiformis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.071 | - |
Urea | pH 7.0, 25°C, soluble enzyme | Canavalia ensiformis |  |
| 0.165 | - |
Urea | pH 7.0, 25°C, urease encapsulated in chitosan nickel ferrite beads | Canavalia ensiformis | |
| 0.168 | - |
Urea | pH 7.0, 25°C, urease encapsulated in chitosan-magnetite beads | Canavalia ensiformis | |
| 0.172 | - |
Urea | pH 7.0, 25°C, urease encapsulated in chitosan-coated alginate-magnetite | Canavalia ensiformis | |
| 0.181 | - |
Urea | pH 7.0, 25°C, urease encapsulated in chitosan-coated alginate-nickel | Canavalia ensiformis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| urea + H2O | Canavalia ensiformis | - |
CO2 + 2 NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Canavalia ensiformis | P07374 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| urea + H2O | - |
Canavalia ensiformis | CO2 + 2 NH3 | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Canavalia ensiformis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
activity of free urease significantly decreases above 35°C | Canavalia ensiformis |
| 70 | - |
urease-encapsulated magnetic beads protect the stability of 30-45% relative activity at 70°C | Canavalia ensiformis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 8 | urease encapsulated in chitosan-coated alginate-magnetite and chitosan-coated alginate-nickel ferrite beads | Canavalia ensiformis |
| 6 | 7.5 | urease encapsulated in chitosan nickel ferrite beads | Canavalia ensiformis |
| 6.5 | 7.5 | urease encapsulated in chitosan-magnetite beads | Canavalia ensiformis |
| 7.5 | - |
soluble enzyme | Canavalia ensiformis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
a significant increase in pH stability is observed for urease-encapsulated magnetic beads compared with the free urease for both acidic and alkaline medium | Canavalia ensiformis |
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