Literature summary for 3.5.1.5 extracted from

Mulinari, F.; Becker-Ritt, A.B.; Demartini, D.R.; Ligabue-Braun, R.; Staniscuaski, F.; Verli, H.; Fragoso, R.R.; Schroeder, E.K.; Carlini, C.R.; Grossi-de-Sa, M.F.
Characterization of JBURE-IIb isoform of Canavalia ensiformis (L.) DC urease (2011), Biochim. Biophys. Acta, 1814, 1758-1768.

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Cloned(Commentary)

Cloned (Comment)	Organism
His-tagged version expressed in Escherichia coli BL21 STAR (DE3)	Canavalia ensiformis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ni2+	metallocenter, nickel-dependent	Canavalia ensiformis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
90000	-	x * 90000 (SDS-PAGE), 90059.6 (predicted sequence)	Canavalia ensiformis

Organism

Organism	UniProt	Comment	Textmining
Canavalia ensiformis	E6Y5X0	-	-

Purification (Commentary)

Purification (Comment)	Organism
immobilized metal ion affinity chromatography (Ni2+), purified protein without enzymatic activity, recombinant Escherichia coli cells positive tested for urease activity in a urea segregation agar assay	Canavalia ensiformis

Subunits

Subunits	Comment	Organism
?	x * 90000 (SDS-PAGE), 90059.6 (predicted sequence)	Canavalia ensiformis

Synonyms

Synonyms	Comment	Organism
urease JBURE-IIb	-	Canavalia ensiformis

pI Value

Organism	Comment	pI Value Maximum	pI Value
Canavalia ensiformis	calculated	-	5.6

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Release 2023.1 (January 2023)