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Literature summary for 3.5.1.5 extracted from

  • Berlicki, L.; Bochno, M.; Grabowiecka, A.; Bialas, A.; Kosikowska, P.; Kafarski, P.
    N-Substituted aminomethanephosphonic and aminomethane-P-methylphosphinic acids as inhibitors of ureases (2012), Amino Acids, 42, 1937-1945.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.5

Inhibitors

Inhibitors Comment Organism Structure
(aminomethyl)methylphosphinic acid
-
 Sporosarcina pasteurii
(aminomethyl)phosphonic acid
-
 Canavalia ensiformis
(aminomethyl)phosphonic acid
-
 Sporosarcina pasteurii
methyl[(methylamino)methyl]phosphinic acid
-
 Canavalia ensiformis
methyl[(methylamino)methyl]phosphinic acid
-
 Sporosarcina pasteurii
[(acetylamino)methyl]methylphosphinic acid
-
 Canavalia ensiformis
[(acetylamino)methyl]methylphosphinic acid
-
 Sporosarcina pasteurii
[(acetylamino)methyl]phosphonic acid
-
 Sporosarcina pasteurii
[(benzylamino)methyl]methylphosphinic acid
-
 Canavalia ensiformis
[(benzylamino)methyl]methylphosphinic acid
-
 Sporosarcina pasteurii
[(dimethylamino)methyl]methylphosphinic acid
-
 Canavalia ensiformis
[(dimethylamino)methyl]methylphosphinic acid
-
 Sporosarcina pasteurii
[(dimethylamino)methyl]phosphonic acid
-
 Canavalia ensiformis
[(dimethylamino)methyl]phosphonic acid
-
 Sporosarcina pasteurii
[(methylamino)methyl]phosphonic acid
-
 Canavalia ensiformis
[(methylamino)methyl]phosphonic acid
-
 Sporosarcina pasteurii
[[benzyl(methyl)amino]methyl]phosphonic acid
-
 Sporosarcina pasteurii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7
-
 Urea estimated value, pH 7.0, 37°C Canavalia ensiformis
17.6
-
 Urea partially purified enzyme, pH 7.0, 37°C Sporosarcina pasteurii

Organism

Organism UniProt Comment Textmining
Canavalia ensiformis
-
-
-
Sporosarcina pasteurii
-
-
-
Sporosarcina pasteurii CCM 2056T
-
-
-

Purification (Commentary)

Purification (Comment) Organism
anion-exchange chromatography, hydrophobic interaction chromatography, affinity chromatography (Cellufine Sulphate) Sporosarcina pasteurii
commercial preparation Canavalia ensiformis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
urea + H2O
-
 Canavalia ensiformis 2 NH3 + CO2
-
 ?
urea + H2O
-
 Sporosarcina pasteurii 2 NH3 + CO2
-
 ?
urea + H2O
-
 Sporosarcina pasteurii CCM 2056T 2 NH3 + CO2
-
 ?

Synonyms

Synonyms Comment Organism
urease
-
 Canavalia ensiformis
urease
-
 Sporosarcina pasteurii

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00062
-
 [(dimethylamino)methyl]methylphosphinic acid pH 7.0, 37°C Sporosarcina pasteurii
0.0037
-
 [(dimethylamino)methyl]methylphosphinic acid pH 7.0, 37°C Canavalia ensiformis
0.013
-
 [(dimethylamino)methyl]phosphonic acid pH 7.0, 37°C Sporosarcina pasteurii
0.018
-
 methyl[(methylamino)methyl]phosphinic acid pH 7.0, 37°C Sporosarcina pasteurii
0.021
-
 [(dimethylamino)methyl]phosphonic acid pH 7.0, 37°C Canavalia ensiformis
0.07
-
 [(methylamino)methyl]phosphonic acid pH 7.0, 37°C Sporosarcina pasteurii
0.072
-
 [(benzylamino)methyl]methylphosphinic acid pH 7.0, 37°C Sporosarcina pasteurii
0.095
-
 [(acetylamino)methyl]methylphosphinic acid pH 7.0, 37°C Sporosarcina pasteurii
0.115
-
 [[benzyl(methyl)amino]methyl]phosphonic acid pH 7.0, 37°C Sporosarcina pasteurii
0.148
-
 [(acetylamino)methyl]phosphonic acid pH 7.0, 37°C Sporosarcina pasteurii
0.204
-
 [(methylamino)methyl]phosphonic acid pH 7.0, 37°C Canavalia ensiformis
0.224
-
 methyl[(methylamino)methyl]phosphinic acid pH 7.0, 37°C Canavalia ensiformis
0.24
-
 (aminomethyl)phosphonic acid pH 7.0, 37°C Canavalia ensiformis
0.295
-
 [(acetylamino)methyl]methylphosphinic acid pH 7.0, 37°C Canavalia ensiformis
0.314
-
 (aminomethyl)phosphonic acid pH 7.0, 37°C Sporosarcina pasteurii
0.34
-
 (aminomethyl)methylphosphinic acid pH 7.0, 37°C Sporosarcina pasteurii
0.571
-
 [(benzylamino)methyl]methylphosphinic acid pH 7.0, 37°C Canavalia ensiformis

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0038
-
 pH 7.0, 37°C Sporosarcina pasteurii [(dimethylamino)methyl]methylphosphinic acid
0.0144
-
 pH 7.0, 37°C Canavalia ensiformis [(dimethylamino)methyl]methylphosphinic acid
0.049
-
 pH 7.0, 37°C Sporosarcina pasteurii [(dimethylamino)methyl]phosphonic acid
0.06
-
 pH 7.0, 37°C Sporosarcina pasteurii methyl[(methylamino)methyl]phosphinic acid
0.082
-
 pH 7.0, 37°C Canavalia ensiformis [(dimethylamino)methyl]phosphonic acid
0.228
-
 pH 7.0, 37°C Sporosarcina pasteurii [(methylamino)methyl]phosphonic acid
0.256
-
 pH 7.0, 37°C Sporosarcina pasteurii [(benzylamino)methyl]methylphosphinic acid
0.258
-
 pH 7.0, 37°C Sporosarcina pasteurii [(acetylamino)methyl]methylphosphinic acid
0.3
-
 pH 7.0, 37°C Sporosarcina pasteurii [[benzyl(methyl)amino]methyl]phosphonic acid
0.432
-
 pH 7.0, 37°C Canavalia ensiformis (aminomethyl)phosphonic acid
0.463
-
 pH 7.0, 37°C Sporosarcina pasteurii [(acetylamino)methyl]phosphonic acid
0.678
-
 pH 7.0, 37°C Canavalia ensiformis [(methylamino)methyl]phosphonic acid
0.7
-
 pH 7.0, 37°C Sporosarcina pasteurii (aminomethyl)phosphonic acid
0.884
-
 pH 7.0, 37°C Canavalia ensiformis methyl[(methylamino)methyl]phosphinic acid
1.1
-
 pH 7.0, 37°C Sporosarcina pasteurii (aminomethyl)methylphosphinic acid
1.455
-
 pH 7.0, 37°C Canavalia ensiformis [(acetylamino)methyl]methylphosphinic acid
1.632
-
 pH 7.0, 37°C Canavalia ensiformis [(benzylamino)methyl]methylphosphinic acid