Application | Comment | Organism |
---|---|---|
medicine | the action of urease in the upper intestinal tract is exploited in a non-invasive urease breath test for the diagnosis of bacterial infections of Helicobacter pylori: 13C- or 14C-labelled urea is ingested, and if the bacterium is present in the stomach, the urea is converted into isotope-labelled carbon dioxide. This is absorbed into the blood and exhaled in the breath, where it is detected by mass spectrometer or scintillation counter | Helicobacter pylori |
Crystallization (Comment) | Organism |
---|---|
crystal structure determined at 2.0 A | Sporosarcina pasteurii |
crystal structure determined at 2.2 A | Klebsiella aerogenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
Urea | pH 8.4, temperature not specified in the publication | Otala lactea | |
0.2 | - |
Urea | range Km: 0.2-0.8 mM, pH 8.0, temperature not specified in the publication | Helicobacter pylori | |
1.03 | - |
Urea | pH 8.2, temperature not specified in the publication | Schizosaccharomyces pombe | |
1.33 | - |
Urea | pH 8.5, temperature not specified in the publication | Aspergillus nidulans | |
1.66 | - |
Urea | pH and temperature not specified in the publication | Staphylococcus leei | |
2 | - |
Urea | pH 4.5, temperature not specified in the publication | Streptococcus mitior | |
2.2 | - |
Urea | pH 8, temperature not specified in the publication | Selenomonas ruminantium | |
2.5 | - |
Urea | pH 6.9, temperature not specified in the publication | Ureaplasma urealyticum | |
2.7 | - |
Urea | pH 2, temperature not specified in the publication | Limosilactobacillus fermentum | |
2.8 | - |
Urea | pH 7.0, 37°C | Klebsiella aerogenes | |
2.8 | - |
Urea | pH 2, temperature not specified in the publication | Limosilactobacillus reuteri | |
2.8 | - |
Urea | pH 7.5, temperature not specified in the publication | Klebsiella aerogenes | |
2.8 | - |
Urea | pH 7.75, temperature not specified in the publication | Klebsiella aerogenes | |
2.9 | - |
Urea | pH 7.0, 38°C | Canavalia ensiformis | |
3 | - |
Urea | pH 8.0, temperature not specified in the publication | Aspergillus niger | |
3 | - |
Urea | pH 4.2, temperature not specified in the publication | Arthrobacter mobilis | |
3 | - |
Urea | pH 7.3, temperature not specified in the publication | Cajanus cajan | |
4.1 | - |
Urea | pH 8.0, temperature not specified in the publication | Coccidioides immitis | |
4.5 | - |
Urea | pH and temperature not specified in the publication | Nitellopsis obtusa | |
5.6 | - |
Urea | pH 7.0, temperature not specified in the publication | Brucella suis | |
9.3 | - |
Urea | pH and temperature not specified in the publication | Providencia stuartii | |
9.5 | - |
Urea | pH 6, temperature not specified in the publication | Staphylococcus saprophyticus | |
10.5 | - |
Urea | pH 7.5, temperature not specified in the publication | Providencia rettgeri | |
12.5 | - |
Urea | pH 7.6, temperature not specified in the publication | Pseudarthrobacter oxydans | |
13 | - |
Urea | pH 7.5, temperature not specified in the publication | Proteus mirabilis | |
17.3 | - |
Urea | pH 8.0, temperature not specified in the publication | Sporosarcina pasteurii | |
32 | - |
Urea | pH 7.0, temperature not specified in the publication | Corynebacterium ammoniagenes |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ni2+ | required for activity | Canavalia ensiformis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
11000 | - |
1 * 61000 + 1 * 11000 + 1 * 14000 composed of three distinct subunits (one large (61 kDa) and two small (11 kDa and 14 kDa)), commonly forming (alpha beta gamma)3 trimers | Sporosarcina pasteurii |
11100 | - |
gamma subunit, method not specified | Klebsiella aerogenes |
11100 | - |
gamma subunit, method not specified | Sporosarcina pasteurii |
11700 | - |
beta subunit, method not specified | Klebsiella aerogenes |
12000 | - |
gamma subunit, method not specified | Staphylococcus leei |
14000 | - |
beta subunit, method not specified | Sporosarcina pasteurii |
20400 | - |
beta subunit, method not specified | Staphylococcus saprophyticus |
21000 | - |
beta subunit, method not specified | Staphylococcus leei |
26500 | - |
beta subunit, method not specified | Helicobacter pylori |
60300 | - |
alpha subunit, method not specified | Klebsiella aerogenes |
61000 | - |
alpha subunit, method not specified | Helicobacter pylori |
61000 | - |
1 * 61000 + 1 * 11000 + 1 * 14000 composed of three distinct subunits (one large (61 kDa) and two small (11 kDa and 14 kDa)), commonly forming (alpha beta gamma)3 trimers | Sporosarcina pasteurii |
61400 | - |
alpha subunit, method not specified | Sporosarcina pasteurii |
65000 | - |
alpha subunit, method not specified | Staphylococcus leei |
72400 | - |
alpha subunit, method not specified | Staphylococcus saprophyticus |
90770 | - |
alpha subunit of jack bean urease without Ni2+, method not specified | Canavalia ensiformis |
113900 | - |
gamma subunit, method not specified | Staphylococcus saprophyticus |
545300 | - |
hexamer with 12 nickel ions included, method not specified | Canavalia ensiformis |
590000 | - |
hexamer with 12 nickel ions included, sedimentation method | Canavalia ensiformis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter mobilis | - |
- |
- |
Aspergillus nidulans | - |
- |
- |
Aspergillus niger | - |
- |
- |
Brucella suis | - |
- |
- |
Cajanus cajan | - |
- |
- |
Canavalia ensiformis | - |
- |
- |
Coccidioides immitis | - |
- |
- |
Corynebacterium ammoniagenes | - |
- |
- |
Helicobacter pylori | - |
- |
- |
Klebsiella | - |
- |
- |
Klebsiella aerogenes | - |
- |
- |
Limosilactobacillus fermentum | - |
- |
- |
Limosilactobacillus reuteri | - |
- |
- |
Nitellopsis obtusa | - |
- |
- |
Otala lactea | - |
- |
- |
Proteus mirabilis | - |
- |
- |
Providencia rettgeri | - |
- |
- |
Providencia stuartii | - |
- |
- |
Pseudarthrobacter oxydans | - |
- |
- |
Pseudomonas | - |
- |
- |
Schizosaccharomyces pombe | - |
- |
- |
Selenomonas ruminantium | - |
- |
- |
Sporosarcina pasteurii | - |
- |
- |
Staphylococcus leei | - |
- |
- |
Staphylococcus saprophyticus | - |
- |
- |
Staphylococcus sp. | - |
- |
- |
Staphylococcus xylosus | - |
- |
- |
Streptococcus mitior | - |
- |
- |
Ureaplasma urealyticum | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.79 | - |
pH 8.4, temperature not specified in the publication | Otala lactea |
60.6 | - |
pH 7.5, temperature not specified in the publication | Providencia rettgeri |
219 | - |
pH 7.6, temperature not specified in the publication | Pseudarthrobacter oxydans |
220 | - |
pH 2, temperature not specified in the publication | Limosilactobacillus fermentum |
230 | - |
pH 4.5, temperature not specified in the publication | Streptococcus mitior |
290 | - |
pH 2, temperature not specified in the publication | Limosilactobacillus reuteri |
540 | - |
pH 7.0, temperature not specified in the publication | Brucella suis |
670 | - |
pH 8.5, temperature not specified in the publication | Aspergillus nidulans |
690 | - |
pH 7.5, temperature not specified in the publication | Klebsiella aerogenes |
700 | - |
pH 8.2, temperature not specified in the publication | Schizosaccharomyces pombe |
730 | - |
pH and temperature not specified in the publication | Staphylococcus leei |
1100 | - |
pH 8, temperature not specified in the publication | Selenomonas ruminantium |
1341 | - |
pH 8.0, temperature not specified in the publication | Aspergillus niger |
1573 | - |
pH 7.2, temperature not specified in the publication | Staphylococcus xylosus |
1700 | - |
pH 8.0, temperature not specified | Helicobacter pylori |
1750 | - |
pH 8.0, temperature not specified in the publication | Coccidioides immitis |
1979 | - |
pH 6, temperature not specified in the publication | Staphylococcus saprophyticus |
2000 | - |
pH 7.5, temperature not specified in the publication | Proteus mirabilis |
2370 | - |
pH 4.2, temperature not specified in the publication | Arthrobacter mobilis |
2500 | - |
pH 7.75, temperature not specified in the publication | Klebsiella aerogenes |
2500 | - |
pH 8.0, temperature not specified in the publication | Sporosarcina pasteurii |
3120 | - |
pH 7.3, temperature not specified in the publication | Cajanus cajan |
3570 | - |
pH 7.0, temperature not specified in the publication | Corynebacterium ammoniagenes |
7100 | - |
pH and temperature not specified in the publication | Providencia stuartii |
180000 | - |
pH 6.9, temperature not specified in the publication | Ureaplasma urealyticum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
urea + H2O | - |
Aspergillus nidulans | CO2 + NH3 | - |
? | |
urea + H2O | - |
Aspergillus niger | CO2 + NH3 | - |
? | |
urea + H2O | - |
Klebsiella aerogenes | CO2 + NH3 | - |
? | |
urea + H2O | - |
Proteus mirabilis | CO2 + NH3 | - |
? | |
urea + H2O | - |
Schizosaccharomyces pombe | CO2 + NH3 | - |
? | |
urea + H2O | - |
Helicobacter pylori | CO2 + NH3 | - |
? | |
urea + H2O | - |
Staphylococcus sp. | CO2 + NH3 | - |
? | |
urea + H2O | - |
Limosilactobacillus fermentum | CO2 + NH3 | - |
? | |
urea + H2O | - |
Selenomonas ruminantium | CO2 + NH3 | - |
? | |
urea + H2O | - |
Ureaplasma urealyticum | CO2 + NH3 | - |
? | |
urea + H2O | - |
Corynebacterium ammoniagenes | CO2 + NH3 | - |
? | |
urea + H2O | - |
Canavalia ensiformis | CO2 + NH3 | - |
? | |
urea + H2O | - |
Providencia rettgeri | CO2 + NH3 | - |
? | |
urea + H2O | - |
Limosilactobacillus reuteri | CO2 + NH3 | - |
? | |
urea + H2O | - |
Sporosarcina pasteurii | CO2 + NH3 | - |
? | |
urea + H2O | - |
Streptococcus mitior | CO2 + NH3 | - |
? | |
urea + H2O | - |
Cajanus cajan | CO2 + NH3 | - |
? | |
urea + H2O | - |
Providencia stuartii | CO2 + NH3 | - |
? | |
urea + H2O | - |
Coccidioides immitis | CO2 + NH3 | - |
? | |
urea + H2O | - |
Pseudarthrobacter oxydans | CO2 + NH3 | - |
? | |
urea + H2O | - |
Otala lactea | CO2 + NH3 | - |
? | |
urea + H2O | - |
Staphylococcus saprophyticus | CO2 + NH3 | - |
? | |
urea + H2O | - |
Staphylococcus xylosus | CO2 + NH3 | - |
? | |
urea + H2O | - |
Staphylococcus leei | CO2 + NH3 | - |
? | |
urea + H2O | - |
Brucella suis | CO2 + NH3 | - |
? | |
urea + H2O | - |
Pseudomonas | CO2 + NH3 | - |
? | |
urea + H2O | - |
Klebsiella | CO2 + NH3 | - |
? | |
urea + H2O | - |
Arthrobacter mobilis | CO2 + NH3 | - |
? | |
urea + H2O | - |
Nitellopsis obtusa | CO2 + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dodecamer | ((alpha beta)3)4 dodecameric complex, method not specified | Helicobacter pylori |
homodimer | method not specified | Schizosaccharomyces pombe |
homohexamer | 6 * 90770, without Ni ions, method not specified | Canavalia ensiformis |
trimer | (alpha beta gamma)4 trimer, method not specified | Staphylococcus saprophyticus |
trimer | (alpha beta gamma)5 trimer. Method not specified | Staphylococcus leei |
trimer | 1 * 61000 + 1 * 11000 + 1 * 14000 composed of three distinct subunits (one large (61 kDa) and two small (11 kDa and 14 kDa)), commonly forming (alpha beta gamma)3 trimers | Sporosarcina pasteurii |
trimer | composed of three distinct subunits, one large (60 kDa) and two small (11 kDa), commonly forming (alpha beta gamma)3 trimers | Klebsiella aerogenes |
Synonyms | Comment | Organism |
---|---|---|
urease | - |
Aspergillus nidulans |
urease | - |
Aspergillus niger |
urease | - |
Klebsiella aerogenes |
urease | - |
Proteus mirabilis |
urease | - |
Schizosaccharomyces pombe |
urease | - |
Helicobacter pylori |
urease | - |
Staphylococcus sp. |
urease | - |
Limosilactobacillus fermentum |
urease | - |
Selenomonas ruminantium |
urease | - |
Ureaplasma urealyticum |
urease | - |
Corynebacterium ammoniagenes |
urease | - |
Canavalia ensiformis |
urease | - |
Providencia rettgeri |
urease | - |
Limosilactobacillus reuteri |
urease | - |
Sporosarcina pasteurii |
urease | - |
Streptococcus mitior |
urease | - |
Cajanus cajan |
urease | - |
Providencia stuartii |
urease | - |
Coccidioides immitis |
urease | - |
Pseudarthrobacter oxydans |
urease | - |
Otala lactea |
urease | - |
Staphylococcus saprophyticus |
urease | - |
Staphylococcus xylosus |
urease | - |
Staphylococcus leei |
urease | - |
Brucella suis |
urease | - |
Pseudomonas |
urease | - |
Klebsiella |
urease | - |
Arthrobacter mobilis |
urease | - |
Nitellopsis obtusa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Klebsiella aerogenes |
38 | - |
assay at | Canavalia ensiformis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3500 | - |
Urea | pH 7.0, 37°C | Klebsiella aerogenes | |
5913 | - |
Urea | pH 7.0, 38°C | Canavalia ensiformis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
2 | - |
- |
Limosilactobacillus fermentum |
2 | - |
- |
Limosilactobacillus reuteri |
4.2 | - |
- |
Arthrobacter mobilis |
4.5 | - |
- |
Streptococcus mitior |
6 | - |
- |
Staphylococcus saprophyticus |
7 | - |
- |
Corynebacterium ammoniagenes |
7 | - |
- |
Brucella suis |
7 | - |
assay at | Klebsiella aerogenes |
7 | - |
assay at | Canavalia ensiformis |
7.2 | - |
- |
Staphylococcus xylosus |
7.3 | - |
- |
Cajanus cajan |
7.5 | - |
- |
Klebsiella aerogenes |
7.5 | - |
- |
Proteus mirabilis |
7.5 | - |
- |
Providencia rettgeri |
7.6 | - |
- |
Pseudarthrobacter oxydans |
7.75 | - |
- |
Klebsiella aerogenes |
8 | - |
- |
Aspergillus niger |
8 | - |
- |
Helicobacter pylori |
8 | - |
- |
Selenomonas ruminantium |
8 | - |
- |
Sporosarcina pasteurii |
8 | - |
- |
Coccidioides immitis |
8.2 | - |
- |
Schizosaccharomyces pombe |
8.5 | - |
- |
Aspergillus nidulans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.9 | 7.5 | optimal activity | Ureaplasma urealyticum |
8.4 | 9 | optimal activity | Otala lactea |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | - |
irreversibly inactivated below pH 5 | Helicobacter pylori |
General Information | Comment | Organism |
---|---|---|
physiological function | the bacterium typically colonizes the mucosal lining of the stomach, where the increase in pH of the strongly acidic environment allows the bacterium that requires pH 6-8 to grow, to persist in the hostile conditions. Concomitantly it incurs damage to the host tissue, thereby giving rise to gastritis and gastroduodenal ulcers. The damaging factors are ammonia and monochloramine | Helicobacter pylori |
physiological function | ureolytic bacterium responsible for the formation of urinary stones in human | Proteus mirabilis |
physiological function | ureolytic bacterium responsible for the formation of urinary stones in human | Staphylococcus sp. |
physiological function | ureolytic bacterium responsible for the formation of urinary stones in human | Ureaplasma urealyticum |
physiological function | ureolytic bacterium responsible for the formation of urinary stones in human | Pseudomonas |
physiological function | ureolytic bacterium responsible for the formation of urinary stones in human | Klebsiella |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1250 | - |
Urea | pH 7.0, 37°C | Klebsiella aerogenes | |
2000 | - |
Urea | pH 7.0, 38°C | Canavalia ensiformis |