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Literature summary for 3.5.1.5 extracted from
- Immobilization of soybean (Glycine max) urease on alginate and chitosan beads showing improved stability: Analytical applications (2009), J. Mol. Catal. B, 58, 138-145.
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| biotechnology | soybean (Glycine max) urease is immobilized on alginate and chitosan beads and shows improved stability. This could have a potential role in haemodialysis machines | Glycine max |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.7 | - |
Urea | soluble urease, Vmax: 285 micromol/min/mg | Glycine max |  |
| 3.92 | - |
Urea | chitosan-immobilized urease, Vmax: 265 micromol/min/mg | Glycine max | |
| 5.88 | - |
Urea | alginate-immobilized urease, Vmax: 282 micromol/min/mg | Glycine max | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Glycine max | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| urea + H2O | - |
Glycine max | CO2 + NH3 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| urease | - |
Glycine max |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
soluble urease | Glycine max |
| 75 | - |
chitosan-immobilized urease | Glycine max |
| 80 | - |
alginate-immobilized urease | Glycine max |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | - |
immobilized urease shows improved stability when stored at 4°C and t1/2 of urease is found to be 19 days, 80 days and 121 days, respectively for soluble, alginate and chitosan ureases | Glycine max |
| 75 | - |
immobilized urease shows improved stability at 75°C. Soluble urease t1/2: 12 min, chitosan-immobilized urease t1/2: 58 min, alginate-immobilized urease t1/2: 43 min | Glycine max |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.2 | - |
alginate-immobilized urease | Glycine max |
| 7 | - |
soluble urease | Glycine max |
| 7.9 | - |
chitosan-immobilized urease | Glycine max |
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