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Literature summary for 3.5.1.5 extracted from

  • Bhagat, N.; Virdi, J.
    Molecular and biochemical characterization of urease and survival of Yersinia enterocolitica biovar 1A in acidic pH in vitro (2009), BMC Microbiol., 9, 262.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.5

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7
-
 Urea pH 5.5, 65°C, Vmax: 7.29 micromol of ammonia/min/mg protein Yersinia enterocolitica

Metals/Ions

Metals/Ions Comment Organism Structure
Ni2+ activity is dependent on Ni in the medium Yersinia enterocolitica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
545000
-
 native PAGE Yersinia enterocolitica

Organism

Organism UniProt Comment Textmining
Yersinia enterocolitica
-
 biovar 1A strain
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
urea + H2O
-
 Yersinia enterocolitica CO2 + 2 NH3
-
 ?

Synonyms

Synonyms Comment Organism
urease
-
 Yersinia enterocolitica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
 Yersinia enterocolitica biovar 1A grown at 28°C (optimum temperature for growth) exhibits higher urease activity than that grown at 37°C Yersinia enterocolitica
37
-
 assay at Yersinia enterocolitica
65
-
 optimal assay temperature Yersinia enterocolitica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Yersinia enterocolitica

pH Range

pH Minimum pH Maximum Comment Organism
4 7
-
 Yersinia enterocolitica

pH Stability

pH Stability pH Stability Maximum Comment Organism
2.5
-
 the amount of viable Yersinia enterocolitica biovar 1A decreases significantly when incubated at pH 2.5 for 2 h. No decrease is observed at this pH in the presence of urea Yersinia enterocolitica