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Literature summary for 3.5.1.5 extracted from

  • Yang, L.Q.; Wang, S.H.; Tian, Y.P.
    Purification, properties, and application of a novel acid urease from Enterobacter sp. (2010), Appl. Biochem. Biotechnol., 160, 303-313.
    View publication on PubMed
Search for more literature for 3.5.1.5

Application

Application Comment Organism
food industry the enzyme is applicable to elimination of urea in Chinese rice wine Enterobacter sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0195
-
 Urea
-
 Enterobacter sp.
0.0195
-
 Urea 35°C, pH 4.5 Enterobacter sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
72000
-
 6 * 72000, SDS-PAGE Enterobacter sp.
430000
-
 gel filtration Enterobacter sp.

Organism

Organism UniProt Comment Textmining
Enterobacter sp.
-
-
-
Enterobacter sp.
-
 R-SYB082
-
Enterobacter sp. R-SYB082
-
-
-
Enterobacter sp. R-SYB082
-
 R-SYB082
-

Purification (Commentary)

Purification (Comment) Organism
-
 Enterobacter sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
101.7
-
-
 Enterobacter sp.
109
-
-
 Enterobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
urea + H2O
-
 Enterobacter sp. CO2 + NH3
-
 ?
urea + H2O
-
 Enterobacter sp. R-SYB082 CO2 + NH3
-
 ?
urea + H2O
-
 Enterobacter sp. CO2 + 2 NH3
-
 ?
urea + H2O
-
 Enterobacter sp. R-SYB082 CO2 + 2 NH3
-
 ?

Subunits

Subunits Comment Organism
hexamer 6 * 72000, SDS-PAGE Enterobacter sp.
homohexamer 6 * 72000, SDS-PAGE Enterobacter sp.

Synonyms

Synonyms Comment Organism
urease
-
 Enterobacter sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Enterobacter sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 75 25°C: about 80% of maximal activity, 75°C: about 45% of maximal activity Enterobacter sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 30 min, stable Enterobacter sp.
50
-
 very stable below 50°C Enterobacter sp.
55
-
 30 min, 15% loss of activity Enterobacter sp.
60
-
 30 min, about 25% loss of activity Enterobacter sp.
70
-
 30 min, about 55% loss of activity Enterobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
 Enterobacter sp.

pH Range

pH Minimum pH Maximum Comment Organism
3 7 pH 3.0: about 65% of maximal activity, pH 7.0: anout 75% of maximal activity Enterobacter sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 6
-
 Enterobacter sp.
4 6 35°C, 30 min, stable Enterobacter sp.