General Stability | Organism |
---|---|
the immobilized enzyme is more stable in wet condition than in semi-dry condition and storing it at 4°C is more favourable over 27°C. There is a tremendous decrease in activity when the immobilized urease is not stored in buffer (i.e. semi-dry condition) and storing it at 4°C improves the longevity. Both the alkyl and arylamine-urease in wet condition stored at 4°C are further reused (30times) and the residual activity are 40% and 20%, respectively | Cajanus cajan |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.35 | - |
Urea | soluble enzyme | Cajanus cajan | |
3.43 | - |
Urea | arylamine glass-immobilized urease | Cajanus cajan | |
3.55 | - |
Urea | alkylamine glass-immobilized urease | Cajanus cajan |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cajanus cajan | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Cajanus cajan |
Storage Stability | Organism |
---|---|
4°C, 70 days, soluble enzyme loses 90% of its activity, alkylamine-immobilized enzyme loses 15% of the initial activity, the arylamine-immobilized enzyme loses 17% of its activity | Cajanus cajan |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
urea + H2O | - |
Cajanus cajan | CO2 + NH3 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
47 | - |
soluble enzyme | Cajanus cajan |
77 | - |
urease coupled to the alkylamine glass or arylamine glass | Cajanus cajan |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
77 | - |
15 min, soluble urease has a residual activity of 22%, alkylamine glass-immobilized enzyme and arylamine glass-immobilized urease retain 96% and 98%, respectively. Even after 90 min of incubation immobilized urease retains 50% of activity | Cajanus cajan |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
alkylamine glass-immobilized enzyme | Cajanus cajan |
7 | - |
arylamine glass-immobilized enzyme | Cajanus cajan |
7.3 | - |
soluble enzyme | Cajanus cajan |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3.5 | - |
22°C, 12 h, soluble enzyme retains 5% of its original activity, alkylamine glass-immobilized enzyme and arylamine glass immobilized urease retain 37% and 38%, respectively | Cajanus cajan |