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Literature summary for 3.5.1.5 extracted from
- Development of a large-scale HPLC-based purification for the urease from Staphylococcus leei and determination of subunit structure (2004), Protein Expr. Purif., 34, 111-117.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.66 | - |
Urea | pH 6.8, 37°C | Staphylococcus leei |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 12000 | - |
(alpha,beta,gamma)5, 5 * 65000 + 5 * 21000 + 5 * 12000, SDS-PAGE | Staphylococcus leei |
| 21000 | - |
(alpha,beta,gamma)5, 5 * 65000 + 5 * 21000 + 5 * 12000, SDS-PAGE | Staphylococcus leei |
| 65000 | - |
(alpha,beta,gamma)5, 5 * 65000 + 5 * 21000 + 5 * 12000, SDS-PAGE | Staphylococcus leei |
| 480000 | - |
gel filtration | Staphylococcus leei |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Staphylococcus leei | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| large-scale HPLC-based | Staphylococcus leei |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 730 | - |
- |
Staphylococcus leei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| urea + H2O | - |
Staphylococcus leei | CO2 + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| pentadecamer | (alpha,beta,gamma)5, 5 * 65000 + 5 * 21000 + 5 * 12000, SDS-PAGE | Staphylococcus leei |
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Release 2023.1 (January 2023)
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