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Literature summary for 3.5.1.5 extracted from
- Characterization of urease from the phototrophic bacterium Rhodobacter capsulatus E1F1 (1993), Curr. Microbiol., 27, 119-123.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | 5 mM, 67% inhibition | Rhodobacter capsulatus |  |
| cyanide | 1.0 mM, 32% inhibition | Rhodobacter capsulatus | |
| EDTA | 5 mM, 70% inhibition | Rhodobacter capsulatus | |
| hydroxamic acid | 1.0 mM, 64% inhibition | Rhodobacter capsulatus | |
| p-hydroxymercuribenzoate | 0.5 mM, 90% inhibition | Rhodobacter capsulatus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.28 | - |
Urea | - |
Rhodobacter capsulatus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Rhodobacter capsulatus | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 180000 | - |
sucrose density gradient centrifugation | Rhodobacter capsulatus |
| 185000 | - |
gel filtration | Rhodobacter capsulatus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| urea + H2O | Rhodobacter capsulatus | NH4+ inhibits urease expression | ? | - |
? | |
| urea + H2O | Rhodobacter capsulatus E1F1 | NH4+ inhibits urease expression | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodobacter capsulatus | - |
- |
- |
| Rhodobacter capsulatus E1F1 | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Rhodobacter capsulatus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| urea + H2O | - |
Rhodobacter capsulatus | CO2 + NH3 | - |
? | |
| urea + H2O | - |
Rhodobacter capsulatus E1F1 | CO2 + NH3 | - |
? | |
| urea + H2O | NH4+ inhibits urease expression | Rhodobacter capsulatus | ? | - |
? | |
| urea + H2O | NH4+ inhibits urease expression | Rhodobacter capsulatus E1F1 | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Rhodobacter capsulatus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Rhodobacter capsulatus |
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