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Literature summary for 3.5.1.49 extracted from
- A novel cysteine carbamoyl-switch is responsible for the inhibition of formamidase, a nitrilase superfamily member (2019), Arch. Biochem. Biophys., 662, 151-159 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene amiF, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 (DE3) | Bacillus cereus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| purified His-tagged enzyme in complex with acetate or urea, mixing of 13 mg/ml protein in 20 mM Tris-HCl, pH 8.0, with PEG448-49, 250 mM urea, pH 4.0, and 20% v/v glycerol in capillaries using the counter-diffusion technique, 20°C, X-ray diffraction structure determination and analysis at 1.78 A and 2.15 A resolution, respectively | Bacillus cereus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Urea | inhibition kinetics and effects on enzyme structure, overview | Bacillus cereus |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| formamide + H2O | Helicobacter pylori | - |
formate + NH3 | - |
? | |
| formamide + H2O | Bacillus cereus | - |
formate + NH3 | - |
? | |
| formamide + H2O | Bacillus cereus ATCC 14579 | - |
formate + NH3 | - |
? | |
| formamide + H2O | Bacillus cereus NRRL B-3711 | - |
formate + NH3 | - |
? | |
| formamide + H2O | Bacillus cereus NCIMB 9373 | - |
formate + NH3 | - |
? | |
| formamide + H2O | Bacillus cereus CECT 148 | - |
formate + NH3 | - |
? | |
| formamide + H2O | Bacillus cereus DSM 31 | - |
formate + NH3 | - |
? | |
| formamide + H2O | Helicobacter pylori ATCC 700392 | - |
formate + NH3 | - |
? | |
| formamide + H2O | Bacillus cereus NBRC 15305 | - |
formate + NH3 | - |
? | |
| formamide + H2O | Helicobacter pylori 26695 | - |
formate + NH3 | - |
? | |
| formamide + H2O | Bacillus cereus JCM 2152 | - |
formate + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus cereus | P59701 | - |
- |
| Bacillus cereus ATCC 14579 | P59701 | - |
- |
| Bacillus cereus CECT 148 | P59701 | - |
- |
| Bacillus cereus DSM 31 | P59701 | - |
- |
| Bacillus cereus JCM 2152 | P59701 | - |
- |
| Bacillus cereus NBRC 15305 | P59701 | - |
- |
| Bacillus cereus NCIMB 9373 | P59701 | - |
- |
| Bacillus cereus NRRL B-3711 | P59701 | - |
- |
| Helicobacter pylori | O25836 | - |
- |
| Helicobacter pylori 26695 | O25836 | - |
- |
| Helicobacter pylori ATCC 700392 | O25836 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography and gel filtration | Bacillus cereus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| formamide + H2O | - |
Helicobacter pylori | formate + NH3 | - |
? | |
| formamide + H2O | - |
Bacillus cereus | formate + NH3 | - |
? | |
| formamide + H2O | - |
Bacillus cereus ATCC 14579 | formate + NH3 | - |
? | |
| formamide + H2O | - |
Bacillus cereus NRRL B-3711 | formate + NH3 | - |
? | |
| formamide + H2O | - |
Bacillus cereus NCIMB 9373 | formate + NH3 | - |
? | |
| formamide + H2O | - |
Bacillus cereus CECT 148 | formate + NH3 | - |
? | |
| formamide + H2O | - |
Bacillus cereus DSM 31 | formate + NH3 | - |
? | |
| formamide + H2O | - |
Helicobacter pylori ATCC 700392 | formate + NH3 | - |
? | |
| formamide + H2O | - |
Bacillus cereus NBRC 15305 | formate + NH3 | - |
? | |
| formamide + H2O | - |
Helicobacter pylori 26695 | formate + NH3 | - |
? | |
| formamide + H2O | - |
Bacillus cereus JCM 2152 | formate + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | enzyme structure analysis by dynamic light scattering, circular dichroism, gel filtration, and mass spectrometry | Bacillus cereus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AmiF | - |
Helicobacter pylori |
| AmiF | - |
Bacillus cereus |
| BceAmiF | - |
Bacillus cereus |
| HpyAmiF | - |
Helicobacter pylori |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Bacillus cereus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
assay at | Bacillus cereus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme is a nitrilase superfamily member | Helicobacter pylori |
| evolution | the enzyme is a nitrilase superfamily member | Bacillus cereus |
| additional information | a cysteine carbamoyl-switch is responsible for the inhibition of formamidase. Structure comparisons of Bacillus cereus AmiF and HpyAmiF | Helicobacter pylori |
| additional information | a cysteine carbamoyl-switch is responsible for the inhibition of formamidase. Structure comparisons of BceAmiF and Helicobacter pylori AmiF | Bacillus cereus |
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