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Literature summary for 3.5.1.46 extracted from

  • Hatanaka, H.S.; Fujiyama, K.; Negoro, S.; Urabe, I.; Okada, H.
    Alteration of catalytic function of 6-aminohexanoate-dimer hydrolase by site-directed mutagenesis (1991), J. Ferment. Bioeng., 71, 191-193.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Flavobacterium sp.

Engineering

Protein Variants Comment Organism
D181N mutant with increased Km and decreased activity Flavobacterium sp.
D181E mutant with increased Km and decreased activity Flavobacterium sp.
D181K
-
Flavobacterium sp.
D181H
-
Flavobacterium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
80
-
N-(6-aminohexanoyl)-6-aminohexanoate mutation D181N Flavobacterium sp.
220
-
N-(6-aminohexanoyl)-6-aminohexanoate mutation D181E Flavobacterium sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
intracellular
-
Flavobacterium sp.
-
-

Organism

Organism UniProt Comment Textmining
Flavobacterium sp.
-
-
-
Flavobacterium sp. KI72
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography techniques, recombinant enzyme Flavobacterium sp.

Source Tissue

Source Tissue Comment Organism Textmining
cell culture nylB gene, enzyme EII Flavobacterium sp.
-
cell culture nylB' gene, enzyme EII' Flavobacterium sp.
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-(6-aminohexanoyl)-6-aminohexanoate + H2O mutant protein D181N 20fold less active, mutant D181E 200fold less active Flavobacterium sp. 6-aminohexanoate + 6-aminohexanoate
-
?
N-(6-aminohexanoyl)-6-aminohexanoate + H2O mutant protein D181N 20fold less active, mutant D181E 200fold less active Flavobacterium sp. KI72 6-aminohexanoate + 6-aminohexanoate
-
?

Synonyms

Synonyms Comment Organism
EII
-
Flavobacterium sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6
-
N-(6-aminohexanoyl)-6-aminohexanoate single mutation D181E Flavobacterium sp.
2.7
-
N-(6-aminohexanoyl)-6-aminohexanoate single mutation D181N Flavobacterium sp.
8.9
-
N-(6-aminohexanoyl)-6-aminohexanoate EII without mutation Flavobacterium sp.