Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.1.46 extracted from

  • Fujiyama, K.; Zhang, Y.Z.; Negoro, S.; Urabe, I.; Okada, H.
    Characterization of hybrid enzymes between 6-aminohexanoate-dimer hydrolase and its analogues protein (1991), J. Ferment. Bioeng., 71, 298-302.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Flavobacterium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
20
-
N-(6-aminohexanoyl)-6-aminohexanoate for EII without mutation, substitution of Asp181 to Asn or Glu affects substrate binding Flavobacterium sp.
15
-
N-(6-aminohexanoyl)-6-aminohexanoate
-
Flavobacterium sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
intracellular
-
Flavobacterium sp.
-
-

Organism

Organism UniProt Comment Textmining
Flavobacterium sp.
-
-
-
Flavobacterium sp. KI72
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography techniques, recombinant enzyme Flavobacterium sp.

Source Tissue

Source Tissue Comment Organism Textmining
cell culture nylB gene, enzyme EII Flavobacterium sp.
-
cell culture nylB' gene, enzyme EII' Flavobacterium sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4
-
-
Flavobacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-(6-aminohexanoyl)-6-aminohexanoate + H2O specific acitvity for EII' 0.5% that of EII Flavobacterium sp. 6-aminohexanoate + 6-aminohexanoate
-
?
N-(6-aminohexanoyl)-6-aminohexanoate + H2O specific acitvity for EII' 0.5% that of EII Flavobacterium sp. KI72 6-aminohexanoate + 6-aminohexanoate
-
?
N-(4-aminobutyryl)-4-aminobutyric acid + H2O very low activity for EII Flavobacterium sp. 4-aminobutyric acid + 4-aminobutyric acid
-
?
N-(4-aminobutyryl)-4-aminobutyric acid + H2O very low activity for EII Flavobacterium sp. KI72 4-aminobutyric acid + 4-aminobutyric acid
-
?
N-(6-aminohexanoyl)-4-aminobutyric acid + H2O low activity for EII Flavobacterium sp. 6-aminohexanoic acid + 4-aminobutyric acid
-
?
N-(6-aminohexanoyl)-4-aminobutyric acid + H2O low activity for EII Flavobacterium sp. KI72 6-aminohexanoic acid + 4-aminobutyric acid
-
?
N-(6-aminohexanoyl)-8-aminooctanoic acid + H2O 4fold higher activity than for N-(6-aminohexanoyl)-6-aminohexanoate for enzyme EII, EII' poorly active Flavobacterium sp. 6-aminohexanoate + 8-aminooctanoate
-
?
N-(6-aminohexanoyl)-8-aminooctanoic acid + H2O 4fold higher activity than for N-(6-aminohexanoyl)-6-aminohexanoate for enzyme EII, EII' poorly active Flavobacterium sp. KI72 6-aminohexanoate + 8-aminooctanoate
-
?
N-(6-aminohexanoyl)-aniline + H2O highest activity for enzyme EII, EII' low activity Flavobacterium sp. 6-aminohexanoate + aniline
-
?
N-(6-aminohexanoyl)-aniline + H2O highest activity for enzyme EII, EII' low activity Flavobacterium sp. KI72 6-aminohexanoate + aniline
-
?
N-(8-aminooctanoyl)-6-aminohexanoic acid + H2O low activity for enzyme EII, EII' no activity Flavobacterium sp. 8-aminooctanoic acid + 6-aminohexanoic acid
-
?

Synonyms

Synonyms Comment Organism
EII
-
Flavobacterium sp.
EII'
-
Flavobacterium sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
EII most heat-stable Flavobacterium sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
19
-
N-(6-aminohexanoyl)-6-aminohexanoate
-
Flavobacterium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5 9.5 same value for enzymes EII, EII' Flavobacterium sp.