Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Flavobacterium sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
15 | - |
N-(6-aminohexanoyl)-6-aminohexanoate | - |
Flavobacterium sp. | |
20 | - |
N-(6-aminohexanoyl)-6-aminohexanoate | for EII without mutation, substitution of Asp181 to Asn or Glu affects substrate binding | Flavobacterium sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | - |
Flavobacterium sp. | 5622 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Flavobacterium sp. | - |
- |
- |
Flavobacterium sp. KI72 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, chromatography techniques, recombinant enzyme | Flavobacterium sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | nylB gene, enzyme EII | Flavobacterium sp. | - |
cell culture | nylB' gene, enzyme EII' | Flavobacterium sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.4 | - |
- |
Flavobacterium sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-(4-aminobutyryl)-4-aminobutyric acid + H2O | very low activity for EII | Flavobacterium sp. | 4-aminobutyric acid + 4-aminobutyric acid | - |
? | |
N-(4-aminobutyryl)-4-aminobutyric acid + H2O | very low activity for EII | Flavobacterium sp. KI72 | 4-aminobutyric acid + 4-aminobutyric acid | - |
? | |
N-(6-aminohexanoyl)-4-aminobutyric acid + H2O | low activity for EII | Flavobacterium sp. | 6-aminohexanoic acid + 4-aminobutyric acid | - |
? | |
N-(6-aminohexanoyl)-4-aminobutyric acid + H2O | low activity for EII | Flavobacterium sp. KI72 | 6-aminohexanoic acid + 4-aminobutyric acid | - |
? | |
N-(6-aminohexanoyl)-6-aminohexanoate + H2O | specific acitvity for EII' 0.5% that of EII | Flavobacterium sp. | 6-aminohexanoate + 6-aminohexanoate | - |
? | |
N-(6-aminohexanoyl)-6-aminohexanoate + H2O | specific acitvity for EII' 0.5% that of EII | Flavobacterium sp. KI72 | 6-aminohexanoate + 6-aminohexanoate | - |
? | |
N-(6-aminohexanoyl)-8-aminooctanoic acid + H2O | 4fold higher activity than for N-(6-aminohexanoyl)-6-aminohexanoate for enzyme EII, EII' poorly active | Flavobacterium sp. | 6-aminohexanoate + 8-aminooctanoate | - |
? | |
N-(6-aminohexanoyl)-8-aminooctanoic acid + H2O | 4fold higher activity than for N-(6-aminohexanoyl)-6-aminohexanoate for enzyme EII, EII' poorly active | Flavobacterium sp. KI72 | 6-aminohexanoate + 8-aminooctanoate | - |
? | |
N-(6-aminohexanoyl)-aniline + H2O | highest activity for enzyme EII, EII' low activity | Flavobacterium sp. | 6-aminohexanoate + aniline | - |
? | |
N-(6-aminohexanoyl)-aniline + H2O | highest activity for enzyme EII, EII' low activity | Flavobacterium sp. KI72 | 6-aminohexanoate + aniline | - |
? | |
N-(8-aminooctanoyl)-6-aminohexanoic acid + H2O | low activity for enzyme EII, EII' no activity | Flavobacterium sp. | 8-aminooctanoic acid + 6-aminohexanoic acid | - |
? |
Synonyms | Comment | Organism |
---|---|---|
EII | - |
Flavobacterium sp. |
EII' | - |
Flavobacterium sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
EII most heat-stable | Flavobacterium sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
19 | - |
N-(6-aminohexanoyl)-6-aminohexanoate | - |
Flavobacterium sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | 9.5 | same value for enzymes EII, EII' | Flavobacterium sp. |