Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas sp. |
expression in Escherichia coli | Flavobacterium sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diisopropyl fluorophosphate | - |
Flavobacterium sp. | |
diisopropyl fluorophosphate | 80% inhibition at 1 mM | Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
N-(6-aminohexanoyl)-6-aminohexanoate | - |
Pseudomonas sp. | |
15 | - |
N-(6-aminohexanoyl)-6-aminohexanoate | - |
Flavobacterium sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | - |
Pseudomonas sp. | 5622 | - |
intracellular | - |
Flavobacterium sp. | 5622 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
2 * 42000, SDS-PAGE | Pseudomonas sp. |
42000 | - |
2 * 42000, SDS-PAGE | Flavobacterium sp. |
76000 | - |
gel filtration | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Flavobacterium sp. | - |
- |
- |
Flavobacterium sp. KI72 | - |
- |
- |
Pseudomonas sp. | - |
- |
- |
Pseudomonas sp. NK87 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, chromatography techniques, recombinant enzyme | Pseudomonas sp. |
to homogeneity, chromatography techniques, recombinant enzyme | Flavobacterium sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Pseudomonas sp. | - |
cell culture | - |
Flavobacterium sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.7 | - |
purified from parental strain | Pseudomonas sp. |
4 | - |
purified from cloned Escherichia coli strain | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-(6-aminohexanoyl)-6-aminohexanoate + H2O | - |
Pseudomonas sp. | 6-aminohexanoate + 6-aminohexanoate | - |
? | |
N-(6-aminohexanoyl)-6-aminohexanoate + H2O | - |
Flavobacterium sp. | 6-aminohexanoate + 6-aminohexanoate | - |
? | |
N-(6-aminohexanoyl)-6-aminohexanoate + H2O | - |
Flavobacterium sp. KI72 | 6-aminohexanoate + 6-aminohexanoate | - |
? | |
N-(6-aminohexanoyl)-6-aminohexanoate + H2O | - |
Pseudomonas sp. NK87 | 6-aminohexanoate + 6-aminohexanoate | - |
? | |
N-6-aminohexanoate pentamer + H2O | - |
Pseudomonas sp. | 6-aminohexanoate + ? | - |
? | |
N-6-aminohexanoate pentamer + H2O | - |
Flavobacterium sp. | 6-aminohexanoate + ? | - |
? | |
N-6-aminohexanoate pentamer + H2O | - |
Flavobacterium sp. KI72 | 6-aminohexanoate + ? | - |
? | |
N-6-aminohexanoate pentamer + H2O | - |
Pseudomonas sp. NK87 | 6-aminohexanoate + ? | - |
? | |
N-6-aminohexanoate tetramer + H2O | - |
Flavobacterium sp. | 6-aminohexanoate + N-6-aminohexanoate trimer | - |
? | |
N-6-aminohexanoate tetramer + H2O | highest relative activity | Pseudomonas sp. | 6-aminohexanoate + N-6-aminohexanoate trimer | major products, the trimer is subsequently subjected to hydrolysis, digests exogenously linear oligomers from the N-terminus as monomeric units | ? | |
N-6-aminohexanoate tetramer + H2O | - |
Flavobacterium sp. KI72 | 6-aminohexanoate + N-6-aminohexanoate trimer | - |
? | |
N-6-aminohexanoate tetramer + H2O | highest relative activity | Pseudomonas sp. NK87 | 6-aminohexanoate + N-6-aminohexanoate trimer | major products, the trimer is subsequently subjected to hydrolysis, digests exogenously linear oligomers from the N-terminus as monomeric units | ? | |
N-6-aminohexanoate trimer + H2O | - |
Pseudomonas sp. | 6-aminohexanoate + N-(6-aminohexanoyl)-6-aminohexanoate | - |
? | |
N-6-aminohexanoate trimer + H2O | - |
Flavobacterium sp. | 6-aminohexanoate + N-(6-aminohexanoyl)-6-aminohexanoate | - |
? | |
N-6-aminohexanoate trimer + H2O | - |
Flavobacterium sp. KI72 | 6-aminohexanoate + N-(6-aminohexanoyl)-6-aminohexanoate | - |
? | |
N-6-aminohexanoate trimer + H2O | - |
Pseudomonas sp. NK87 | 6-aminohexanoate + N-(6-aminohexanoyl)-6-aminohexanoate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 42000, SDS-PAGE | Pseudomonas sp. |
dimer | 2 * 42000, SDS-PAGE | Flavobacterium sp. |
Synonyms | Comment | Organism |
---|---|---|
EII | - |
Pseudomonas sp. |
EII | - |
Flavobacterium sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Flavobacterium sp. |
48 | - |
- |
Pseudomonas sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
stable up to | Pseudomonas sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9.2 | - |
N-(6-aminohexanoyl)-6-aminohexanoate | - |
Pseudomonas sp. | |
19 | - |
N-(6-aminohexanoyl)-6-aminohexanoate | - |
Flavobacterium sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Pseudomonas sp. |
9 | - |
- |
Flavobacterium sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.8 | 8.5 | stable within | Pseudomonas sp. |