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Literature summary for 3.5.1.46 extracted from

  • Kanagawa, K.; Oishi, M.; Negoro, S.; Urabe, I.; Okada, H.
    Characterization of the 6-aminohexanoate-dimer hydrolase from Pseudomonas sp. NK87 (1993), J. Gen. Microbiol., 139, 787-795.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas sp.
expression in Escherichia coli Flavobacterium sp.

Inhibitors

Inhibitors Comment Organism Structure
diisopropyl fluorophosphate
-
Flavobacterium sp.
diisopropyl fluorophosphate 80% inhibition at 1 mM Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.6
-
N-(6-aminohexanoyl)-6-aminohexanoate
-
Pseudomonas sp.
15
-
N-(6-aminohexanoyl)-6-aminohexanoate
-
Flavobacterium sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
intracellular
-
Pseudomonas sp.
-
-
intracellular
-
Flavobacterium sp.
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
76000
-
gel filtration Pseudomonas sp.
42000
-
2 * 42000, SDS-PAGE Pseudomonas sp.
42000
-
2 * 42000, SDS-PAGE Flavobacterium sp.

Organism

Organism UniProt Comment Textmining
Flavobacterium sp.
-
-
-
Pseudomonas sp.
-
-
-
Flavobacterium sp. KI72
-
-
-
Pseudomonas sp. NK87
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography techniques, recombinant enzyme Pseudomonas sp.
to homogeneity, chromatography techniques, recombinant enzyme Flavobacterium sp.

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Pseudomonas sp.
-
cell culture
-
Flavobacterium sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7
-
purified from parental strain Pseudomonas sp.
4
-
purified from cloned Escherichia coli strain Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-(6-aminohexanoyl)-6-aminohexanoate + H2O
-
Pseudomonas sp. 6-aminohexanoate + 6-aminohexanoate
-
?
N-(6-aminohexanoyl)-6-aminohexanoate + H2O
-
Flavobacterium sp. 6-aminohexanoate + 6-aminohexanoate
-
?
N-(6-aminohexanoyl)-6-aminohexanoate + H2O
-
Flavobacterium sp. KI72 6-aminohexanoate + 6-aminohexanoate
-
?
N-(6-aminohexanoyl)-6-aminohexanoate + H2O
-
Pseudomonas sp. NK87 6-aminohexanoate + 6-aminohexanoate
-
?
N-6-aminohexanoate trimer + H2O
-
Pseudomonas sp. 6-aminohexanoate + N-(6-aminohexanoyl)-6-aminohexanoate
-
?
N-6-aminohexanoate trimer + H2O
-
Flavobacterium sp. 6-aminohexanoate + N-(6-aminohexanoyl)-6-aminohexanoate
-
?
N-6-aminohexanoate trimer + H2O
-
Flavobacterium sp. KI72 6-aminohexanoate + N-(6-aminohexanoyl)-6-aminohexanoate
-
?
N-6-aminohexanoate trimer + H2O
-
Pseudomonas sp. NK87 6-aminohexanoate + N-(6-aminohexanoyl)-6-aminohexanoate
-
?
N-6-aminohexanoate tetramer + H2O
-
Flavobacterium sp. 6-aminohexanoate + N-6-aminohexanoate trimer
-
?
N-6-aminohexanoate tetramer + H2O highest relative activity Pseudomonas sp. 6-aminohexanoate + N-6-aminohexanoate trimer major products, the trimer is subsequently subjected to hydrolysis, digests exogenously linear oligomers from the N-terminus as monomeric units ?
N-6-aminohexanoate tetramer + H2O
-
Flavobacterium sp. KI72 6-aminohexanoate + N-6-aminohexanoate trimer
-
?
N-6-aminohexanoate tetramer + H2O highest relative activity Pseudomonas sp. NK87 6-aminohexanoate + N-6-aminohexanoate trimer major products, the trimer is subsequently subjected to hydrolysis, digests exogenously linear oligomers from the N-terminus as monomeric units ?
N-6-aminohexanoate pentamer + H2O
-
Pseudomonas sp. 6-aminohexanoate + ?
-
?
N-6-aminohexanoate pentamer + H2O
-
Flavobacterium sp. 6-aminohexanoate + ?
-
?
N-6-aminohexanoate pentamer + H2O
-
Flavobacterium sp. KI72 6-aminohexanoate + ?
-
?
N-6-aminohexanoate pentamer + H2O
-
Pseudomonas sp. NK87 6-aminohexanoate + ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 42000, SDS-PAGE Pseudomonas sp.
dimer 2 * 42000, SDS-PAGE Flavobacterium sp.

Synonyms

Synonyms Comment Organism
EII
-
Pseudomonas sp.
EII
-
Flavobacterium sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Flavobacterium sp.
48
-
-
Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
stable up to Pseudomonas sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
9.2
-
N-(6-aminohexanoyl)-6-aminohexanoate
-
Pseudomonas sp.
19
-
N-(6-aminohexanoyl)-6-aminohexanoate
-
Flavobacterium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Pseudomonas sp.
9
-
-
Flavobacterium sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.8 8.5 stable within Pseudomonas sp.