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Literature summary for 3.5.1.42 extracted from
- Characterization and mutational analysis of a nicotinamide mononucleotide deamidase from Agrobacterium tumefaciens showing high thermal stability and catalytic efficiency (2017), PLoS ONE, 12, e0174759 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene cinA, sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli strain Rosetta 2(DE3) | Agrobacterium fabrum |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| crystal structure PDB ID 2A9S | Agrobacterium fabrum |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C32A | site-directed mutagenesis, the mutant shows 28% reduced activity compared to the wild-type enzyme | Agrobacterium fabrum |
| K638A | site-directed mutagenesis, inactive mutant | Agrobacterium fabrum |
| R145A | site-directed mutagenesis, inactive mutant | Agrobacterium fabrum |
| S31A | site-directed mutagenesis, inactive mutant | Agrobacterium fabrum |
| S48A | site-directed mutagenesis, the mutant shows 47% reduced activity compared to the wild-type enzyme | Agrobacterium fabrum |
| T105A | site-directed mutagenesis, the mutant shows 94% reduced activity compared to the wild-type enzyme | Agrobacterium fabrum |
| Y58A | site-directed mutagenesis, inactive mutant | Agrobacterium fabrum |
| Y58F | site-directed mutagenesis, almost inactive mutant | Agrobacterium fabrum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetic at pH 7.5 and 37°C | Agrobacterium fabrum | |
| 0.024 | - |
nicotinamide mononucleotide | pH 7.5, 37°C, recombinant wild-type enzyme | Agrobacterium fabrum |  |
| 0.028 | - |
nicotinamide mononucleotide | pH 7.5, 37°C, recombinant mutant C32A | Agrobacterium fabrum | |
| 0.051 | - |
nicotinamide mononucleotide | pH 7.5, 37°C, recombinant mutant S48A | Agrobacterium fabrum | |
| 0.158 | - |
nicotinamide mononucleotide | pH 7.5, 37°C, recombinant mutant T105A | Agrobacterium fabrum | |
| 1.93 | - |
nicotinamide mononucleotide | pH 7.5, 37°C, recombinant mutant Y58F | Agrobacterium fabrum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 41000 | - |
recombinant enzyme, gel filtration | Agrobacterium fabrum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nicotinamide mononucleotide + H2O | Agrobacterium fabrum | - |
nicotinate mononucleotide + NH3 | - |
? | |
| nicotinamide mononucleotide + H2O | Agrobacterium fabrum C58 | - |
nicotinate mononucleotide + NH3 | - |
? | |
| nicotinamide mononucleotide + H2O | Agrobacterium fabrum ATCC 33970 | - |
nicotinate mononucleotide + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Agrobacterium fabrum | A9CJ26 | i.e. Agrobacterium tumefaciens strain C58 | - |
| Agrobacterium fabrum ATCC 33970 | A9CJ26 | i.e. Agrobacterium tumefaciens strain C58 | - |
| Agrobacterium fabrum C58 | A9CJ26 | i.e. Agrobacterium tumefaciens strain C58 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nicotinamide mononucleotide + H2O | - |
Agrobacterium fabrum | nicotinate mononucleotide + NH3 | - |
? | |
| nicotinamide mononucleotide + H2O | NaMN binding site, structure, overview | Agrobacterium fabrum | nicotinate mononucleotide + NH3 | - |
? | |
| nicotinamide mononucleotide + H2O | - |
Agrobacterium fabrum C58 | nicotinate mononucleotide + NH3 | - |
? | |
| nicotinamide mononucleotide + H2O | NaMN binding site, structure, overview | Agrobacterium fabrum C58 | nicotinate mononucleotide + NH3 | - |
? | |
| nicotinamide mononucleotide + H2O | - |
Agrobacterium fabrum ATCC 33970 | nicotinate mononucleotide + NH3 | - |
? | |
| nicotinamide mononucleotide + H2O | NaMN binding site, structure, overview | Agrobacterium fabrum ATCC 33970 | nicotinate mononucleotide + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 19000, recombinant enzyme, SDS-PAGE | Agrobacterium fabrum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AtCinA | - |
Agrobacterium fabrum |
| CinA | - |
Agrobacterium fabrum |
| nicotinamide mononucleotide deamidase | - |
Agrobacterium fabrum |
| NMN deamidase | - |
Agrobacterium fabrum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
- |
Agrobacterium fabrum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
melting temperature of the recombinant wild-type enzyme is 85°C | Agrobacterium fabrum |
| 65 | 90 | over 60% of maximal activity | Agrobacterium fabrum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
purified recombinant enzyme, half-life is 15 days | Agrobacterium fabrum |
| 40 | - |
purified recombinant enzyme, 24 h, 50% activity remaining | Agrobacterium fabrum |
| 70 | - |
purified recombinant enzyme, 24 h, 14% activity remaining | Agrobacterium fabrum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.5 | - |
nicotinamide mononucleotide | pH 7.5, 37°C, recombinant mutant Y58F | Agrobacterium fabrum | |
| 3.5 | - |
nicotinamide mononucleotide | pH 7.5, 37°C, recombinant mutant T105A | Agrobacterium fabrum | |
| 7.3 | - |
nicotinamide mononucleotide | pH 7.5, 37°C, recombinant mutant C32A | Agrobacterium fabrum | |
| 8.7 | - |
nicotinamide mononucleotide | pH 7.5, 37°C, recombinant wild-type enzyme | Agrobacterium fabrum | |
| 8.7 | - |
nicotinamide mononucleotide | pH 7.5, 37°C, recombinant mutant S48A | Agrobacterium fabrum | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Agrobacterium fabrum |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to the NMN deamidase family | Agrobacterium fabrum |
| additional information | mutational analysis of AtCinA supports the Ser/Lys catalytic dyad mechanism, overview | Agrobacterium fabrum |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.777 | - |
nicotinamide mononucleotide | pH 7.5, 37°C, recombinant mutant Y58F | Agrobacterium fabrum | |
| 22.15 | - |
nicotinamide mononucleotide | pH 7.5, 37°C, recombinant mutant T105A | Agrobacterium fabrum | |
| 170.6 | - |
nicotinamide mononucleotide | pH 7.5, 37°C, recombinant mutant S48A | Agrobacterium fabrum | |
| 260.7 | - |
nicotinamide mononucleotide | pH 7.5, 37°C, recombinant mutant C32A | Agrobacterium fabrum | |
| 362.5 | - |
nicotinamide mononucleotide | pH 7.5, 37°C, recombinant wild-type enzyme | Agrobacterium fabrum | |
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