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Literature summary for 3.5.1.42 extracted from

  • Sanchez-Carron, G.; Martinez-Monino, A.B.; Sola-Carvajal, A.; Takami, H.; Garcia-Carmona, F.; Sanchez-Ferrer, A.
    New insights into the phylogeny and molecular classification of nicotinamide mononucleotide deamidases (2013), PLoS ONE, 8, e82705.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.42

Cloned(Commentary)

Cloned (Comment) Organism
gene pncC, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain Rosetta2 (DE3) Oceanobacillus iheyensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetics Oceanobacillus iheyensis
0.18
-
 beta-nicotinamide D-ribonucleotide recombinant enzyme, pH 7.4, 37°C Oceanobacillus iheyensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47320
-
 2 * 47320, recombinant His6-tagged enzyme, mass spectrometry Oceanobacillus iheyensis
96100
-
 recombinant His6-tagged enzyme, gel filtration Oceanobacillus iheyensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-nicotinamide D-ribonucleotide + H2O Oceanobacillus iheyensis absolute specific for beta-nicotinate D-ribonucleotide + NH3
-
 ?
beta-nicotinamide D-ribonucleotide + H2O Oceanobacillus iheyensis HTE831 absolute specific for beta-nicotinate D-ribonucleotide + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Oceanobacillus iheyensis Q8EQR8 gene pncC
-
Oceanobacillus iheyensis HTE831 Q8EQR8 gene pncC
-

Purification (Commentary)

Purification (Comment) Organism
recombinant C-terminally His6-tagged enzyme from Escherichia coli strain Rosetta2 (DE3) by nickel affinity chromatography and gel filtration Oceanobacillus iheyensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-nicotinamide D-ribonucleotide + H2O absolute specific for Oceanobacillus iheyensis beta-nicotinate D-ribonucleotide + NH3
-
 ?
beta-nicotinamide D-ribonucleotide + H2O absolute specific for Oceanobacillus iheyensis HTE831 beta-nicotinate D-ribonucleotide + NH3
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 47320, recombinant His6-tagged enzyme, mass spectrometry Oceanobacillus iheyensis

Synonyms

Synonyms Comment Organism
nicotinamide mononucleotide deamidase
-
 Oceanobacillus iheyensis
NMN deamidase
-
 Oceanobacillus iheyensis
OiPncC
-
 Oceanobacillus iheyensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
 Oceanobacillus iheyensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 purified recombinant enzyme, pH 7.3, stable up to Oceanobacillus iheyensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.38
-
 beta-nicotinamide D-ribonucleotide recombinant enzyme, pH 7.4, 37°C Oceanobacillus iheyensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
-
 Oceanobacillus iheyensis

pH Range

pH Minimum pH Maximum Comment Organism
5.5 10 the enzyme is active over a broad pH range, with an optimum at pH 7.4, whilst maintaining 90% activity at pH 10.0 Oceanobacillus iheyensis

pH Stability

pH Stability pH Stability Maximum Comment Organism
10
-
 purified recombinant enzyme, 37°C, maintains 61% activity after 21 days Oceanobacillus iheyensis

General Information

General Information Comment Organism
metabolism nicotinamide mononucleotide (NMN) deamidase is one of the key enzymes of the bacterial pyridine nucleotide cycle, PNC Oceanobacillus iheyensis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.1
-
 beta-nicotinamide D-ribonucleotide recombinant enzyme, pH 7.4, 37°C Oceanobacillus iheyensis