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Literature summary for 3.5.1.41 extracted from

  • Quan, G.; Ladd, T.; Duan, J.; Wen, F.; Doucet, D.; Cusson, M.; Krell, P.J.
    Characterization of a spruce budworm chitin deacetylase gene: stage- and tissue-specific expression, and inhibition using RNA interference (2013), Insect Biochem. Mol. Biol., 43, 683-691.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene CfCDA2a, DNA and amino acid sequence determination and analysis, genetic structure, sequence comparisons, quantitative real-time RT-PCR expression analysis Choristoneura fumiferana
gene CfCDA2b, DNA and amino acid sequence determination and analysis, genetic structure, sequence comparisons, quantitative real-time RT-PCR expression analysis Choristoneura fumiferana

Protein Variants

Protein Variants Comment Organism
additional information RNAi-induced depletion of CfCDA2 transcripts, CfCDA2 transcript levels are strongly suppressed in 5thand 6th-instar larvae injected with CfCDA2-specific dsRNA, compared to controls, CfCDA2 transcript levels are 20% and 50% those of controls in injected 5th- and 6th-instar larvae, respectively Choristoneura fumiferana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61000
-
x * 61000, about, isozyme B, sequence calculation Choristoneura fumiferana
62000
-
x * 62000, about, isozyme A, sequence calculation Choristoneura fumiferana

Organism

Organism UniProt Comment Textmining
Choristoneura fumiferana S5YT69 isozyme b; gene CDA2, splice variant isozyme B
-
Choristoneura fumiferana S5Z6S1 isozyme a; gene CDA2, splice variant isozyme A
-

Source Tissue

Source Tissue Comment Organism Textmining
epidermis epidermis of molting larvae and day 0 pupae Choristoneura fumiferana
-
larva
-
Choristoneura fumiferana
-
additional information the signal is absent or faint in the midgut and larval fat body, tissue- and stage-specific expression of the isozyme, overview Choristoneura fumiferana
-

Subunits

Subunits Comment Organism
? x * 61000, about, isozyme B, sequence calculation Choristoneura fumiferana
? x * 62000, about, isozyme A, sequence calculation Choristoneura fumiferana

Synonyms

Synonyms Comment Organism
CDA
-
Choristoneura fumiferana

pI Value

Organism Comment pI Value Maximum pI Value
Choristoneura fumiferana isozyme A, sequence calculation
-
4.6
Choristoneura fumiferana isozyme B, sequence calculation
-
4.6

General Information

General Information Comment Organism
malfunction enzyme silencing results in abnormal molting phenotypes Choristoneura fumiferana
additional information the isoforms differ only in the alternatively spliced region, representing 43 and 37 amino acid residues in isoforms A and B, respectively Choristoneura fumiferana
physiological function the strong expression of CfCDA2 in the epidermis of molting individuals points to an important role of this gene in the molting process of Choristoneura fumiferana Choristoneura fumiferana