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Literature summary for 3.5.1.41 extracted from
- The cyst wall of Entamoeba invadens contains chitosan (deacetylated chitin) (2006), Mol. Biochem. Parasitol., 148, 86-92.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | chemical acetylation of deproteinated cyst walls prior to treatment with a chitinase leads to 3-4fold activation of the enzyme | Entamoeba invadens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| CDA2, phylogenetic analysis, functional expression as His-tagged protein in Saccharomyces cerevisiae strain BY4741 | Saccharomyces cerevisiae |
| CDA2, phylogenetic analysis, functional expression as His-tagged protein in Saccharomyces cerevisiae strain BY4741 | Entamoeba histolytica |
| phylogenetic analysis | Entamoeba invadens |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | activates | Saccharomyces cerevisiae |  |
| Co2+ | activates | Entamoeba histolytica | |
| Co2+ | activates | Entamoeba invadens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29668 | - |
x * 29668, CDA2, amino acid sequence calculation | Entamoeba invadens |
| 32504 | - |
x * 32504, CDA1, amino acid sequence calculation | Entamoeba invadens |
| 34264 | - |
x * 34264, CDA3, amino acid sequence calculation | Entamoeba invadens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chitin + H2O | Saccharomyces cerevisiae | - |
chitosan + acetate | - |
? | |
| chitin + H2O | Entamoeba histolytica | - |
chitosan + acetate | - |
? | |
| chitin + H2O | Entamoeba invadens | - |
chitosan + acetate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Entamoeba histolytica | - |
isozymes CDA1 and CDA2 | - |
| Entamoeba invadens | Q2V982 | CDA3; three isozymes CDA1, CDA2, and CDA3 | - |
| Entamoeba invadens | Q2V983 | CDA2; three isozymes CDA1, CDA2, and CDA3 | - |
| Entamoeba invadens | Q2V984 | CDA1; three isozymes CDA1, CDA2, and CDA3 | - |
| Saccharomyces cerevisiae | - |
isozymes CDA1 and CDA2 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged CDA2 from Saccharomyces cerevisiae strain BY4741 by nickel affinity chromatography | Saccharomyces cerevisiae |
| recombinant His-tagged CDA2 from Saccharomyces cerevisiae strain BY4741 by nickel affinity chromatography | Entamoeba histolytica |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cyst | wall | Entamoeba invadens | - |
| trophozoite | - |
Entamoeba invadens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chitin + H2O | - |
Saccharomyces cerevisiae | chitosan + acetate | - |
? | |
| chitin + H2O | - |
Entamoeba histolytica | chitosan + acetate | - |
? | |
| chitin + H2O | - |
Entamoeba invadens | chitosan + acetate | - |
? | |
| hexa-N-acetyl-chitohexaose + H2O | - |
Saccharomyces cerevisiae | chitohexaose + acetate | - |
? | |
| hexa-N-acetyl-chitohexaose + H2O | - |
Entamoeba histolytica | chitohexaose + acetate | - |
? | |
| penta-N-acetyl-chitopentaose + H2O | - |
Saccharomyces cerevisiae | chitopentaose + acetate | - |
? | |
| penta-N-acetyl-chitopentaose + H2O | - |
Entamoeba histolytica | chitopentaose + acetate | - |
? | |
| tetra-N-acetyl-chitotetraose + H2O | - |
Saccharomyces cerevisiae | chitotetraose + acetate | - |
? | |
| tetra-N-acetyl-chitotetraose + H2O | - |
Entamoeba histolytica | chitotetraose + acetate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 29668, CDA2, amino acid sequence calculation | Entamoeba invadens |
| ? | x * 32504, CDA1, amino acid sequence calculation | Entamoeba invadens |
| ? | x * 34264, CDA3, amino acid sequence calculation | Entamoeba invadens |
| More | CDA1 amino acid sequence and structure modelling | Entamoeba histolytica |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CDA1 | - |
Entamoeba histolytica |
| CDA1 | - |
Entamoeba invadens |
| Cda1p | - |
Saccharomyces cerevisiae |
| CDA2 | - |
Entamoeba histolytica |
| CDA2 | - |
Entamoeba invadens |
| CDA3 | - |
Entamoeba invadens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
assay at | Saccharomyces cerevisiae |
| 50 | - |
assay at | Entamoeba histolytica |
| 50 | - |
assay at | Entamoeba invadens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Saccharomyces cerevisiae |
| 8 | - |
assay at | Entamoeba histolytica |
| 8 | - |
assay at | Entamoeba invadens |
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Release 2023.1 (January 2023)
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