Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.1.41 extracted from

  • Hekmat, O.; Tokuyasu, K.; Withers, S.G.
    Subsite structure of the endo-type chitin deacetylase from a deuteromycete, Colletotrichum lindemuthianum: an investigation using steady-state kinetic analysis and MS (2003), Biochem. J., 374, 369-380.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.41

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.048
-
 GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc pH 8.5, 30°C Colletotrichum lindemuthianum
0.079
-
 GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc pH 8.5, 30°C Colletotrichum lindemuthianum
0.125
-
 GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc pH 8.5, 30°C Colletotrichum lindemuthianum
4.3
-
 GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc pH 8.5, 30°C Colletotrichum lindemuthianum
18
-
 GlcNAc-beta-(1-4)-GlcNAc pH 8.5, 30°C Colletotrichum lindemuthianum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25756
-
 x * 25756, electrospray ionization MS Colletotrichum lindemuthianum

Organism

Organism UniProt Comment Textmining
Colletotrichum lindemuthianum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Colletotrichum lindemuthianum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GlcNAc-beta-(1-4)-GlcNAc + H2O
-
 Colletotrichum lindemuthianum GlcN-beta-(1-4)-GlcNAc + acetate
-
 ?
GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcN-beta-(1-4)-GlcNAc + H2O
-
 Colletotrichum lindemuthianum GlcNAc-beta-(1-4)-GlcN-beta-(1-4)-GlcN-beta-(1-4)-GlcNAc + GlcN-beta-(1-4)-GlcNAc-beta-(1-4)-GlcN-beta-(1-4)-GlcNAc + acetate
-
 ?
GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc + H2O time courses for hydrolysis of the N-acetyl groups are followed spectrometrically Colletotrichum lindemuthianum acetate + GlcN-beta-(1,4)-GlcNAc-beta-(1,4)-GlcNAc + GlcNAc-beta-(1,4)-GlcN-beta-(1,4)-GlcNAc + GlcNAc-beta-(1,4)-GlcNAc-beta-(1,4)-GlcN GlcN-beta-(1,4)-GlcNAc-beta-(1,4)-GlcNAc, GlcNAc-beta-(1,4)-GlcN-beta-(1,4)-GlcNAc and GlcNAc-beta-(1,4)-GlcNAc-beta-(1,4)-GlcN are formed in the proportions 22:50:28 ?
GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc + H2O time courses for hydrolysis of the N-acetyl groups are followed spectrometrically Colletotrichum lindemuthianum GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcN-beta-(1-4)-GlcNAc + acetate
-
 ?
GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc + H2O time courses for hydrolysis of the N-acetyl groups are followed spectrometrically Colletotrichum lindemuthianum acetate + ?
-
 ?
GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc + H2O time courses for hydrolysis of the N-acetyl groups are followed spectrometrically Colletotrichum lindemuthianum acetate + ?
-
 ?

Subunits

Subunits Comment Organism
? x * 25756, electrospray ionization MS Colletotrichum lindemuthianum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.4
-
 GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc pH 8.5, 30°C Colletotrichum lindemuthianum
6
-
 GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc pH 8.5, 30°C Colletotrichum lindemuthianum
7
-
 GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc pH 8.5, 30°C Colletotrichum lindemuthianum
7
-
 GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc pH 8.5, 30°C Colletotrichum lindemuthianum
11
-
 GlcNAc-beta-(1-4)-GlcNAc pH 8.5, 30°C Colletotrichum lindemuthianum