Literature summary for 3.5.1.41 extracted from

  • Tokuyasu, K.; Kaneko, S.; Hayashi, K.; Mori, Y.
    Production of a recombinant chitin deacetylase in the culture medium of Escherichia coli cells (1999), FEBS Lett., 458, 23-26.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Colletotrichum lindemuthianum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0416
-
(N-acetyl-D-glucosamine)5 S12-CDAH Colletotrichum lindemuthianum
0.0406
-
(N-acetyl-D-glucosamine)5 S1-CDA Colletotrichum lindemuthianum
0.0405
-
(N-acetyl-D-glucosamine)5 S1-CDAH Colletotrichum lindemuthianum
0.0446
-
(N-acetyl-D-glucosamine)5 original CDA Colletotrichum lindemuthianum

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Colletotrichum lindemuthianum
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
chitin + H2O Colletotrichum lindemuthianum
-
chitosan + acetate
-
?

Organism

Organism UniProt Comment Textmining
Colletotrichum lindemuthianum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant and original CDA enzymes, to homogeneity Colletotrichum lindemuthianum

Source Tissue

Source Tissue Comment Organism Textmining
culture filtrate
-
Colletotrichum lindemuthianum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycol chitin + H2O
-
Colletotrichum lindemuthianum chitosan + acetate
-
?
chitin + H2O
-
Colletotrichum lindemuthianum chitosan + acetate
-
?
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
-
Colletotrichum lindemuthianum ?
-
?
(N-acetyl-D-glucosamine)5 + H2O
-
Colletotrichum lindemuthianum (GlcN)4GlcNAc + acetate
-
?
(N-acetyl-D-glucosamine)5
-
Colletotrichum lindemuthianum (GlcN)4GlcNAc + acetate
-
?

Subunits

Subunits Comment Organism
? S1-CDA: 24700, S1-CDAH: 25700, S12-CDAH: 26800, original CDA: 24300, SDS-PAGE Colletotrichum lindemuthianum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
21.5
-
(N-acetyl-D-glucosamine)5 S12-CDAH Colletotrichum lindemuthianum
51.6
-
(N-acetyl-D-glucosamine)5 S1-CDA Colletotrichum lindemuthianum
30.3
-
(N-acetyl-D-glucosamine)5 S1-CDAH Colletotrichum lindemuthianum
47.1
-
(N-acetyl-D-glucosamine)5 original CDA Colletotrichum lindemuthianum