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Literature summary for 3.5.1.4 extracted from
- Promiscuous (+)-gamma-lactamase activity of an amidase from nitrile hydratase pathway for efficient synthesis of carbocyclic nucleosides intermediate (2018), Bioorg. Med. Chem. Lett., 28, 1071-1076 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli BL21(DE3) | Rhodococcus erythropolis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.03 | - |
Benzamide | pH and temperature not specified in the publication | Rhodococcus erythropolis |  |
| 19 | - |
acetamide | pH and temperature not specified in the publication | Rhodococcus erythropolis | |
| 24.1 | - |
Acrylamide | pH and temperature not specified in the publication | Rhodococcus erythropolis | |
| 34.2 | - |
Propionamide | pH and temperature not specified in the publication | Rhodococcus erythropolis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodococcus erythropolis | - |
- |
- |
| Rhodococcus erythropolis R4 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rhodococcus erythropolis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetamide + H2O | - |
Rhodococcus erythropolis | acetate + NH3 | - |
? | |
| acetamide + H2O | - |
Rhodococcus erythropolis R4 | acetate + NH3 | - |
? | |
| acrylamide + H2O | - |
Rhodococcus erythropolis | acrylate + NH3 | - |
? | |
| acrylamide + H2O | - |
Rhodococcus erythropolis R4 | acrylate + NH3 | - |
? | |
| benzamide + H2O | - |
Rhodococcus erythropolis | benzoate + NH3 | - |
? | |
| benzamide + H2O | - |
Rhodococcus erythropolis R4 | benzoate + NH3 | - |
? | |
| propionamide + H2O | - |
Rhodococcus erythropolis | propionate + NH3 | - |
? | |
| propionamide + H2O | - |
Rhodococcus erythropolis R4 | propionate + NH3 | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Rhodococcus erythropolis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
1 h, the enzyme maintains 100% of its original activity when incubated for 1 h | Rhodococcus erythropolis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.1 | - |
Acrylamide | pH and temperature not specified in the publication | Rhodococcus erythropolis | |
| 20.2 | - |
acetamide | pH and temperature not specified in the publication | Rhodococcus erythropolis | |
| 155.2 | - |
Propionamide | pH and temperature not specified in the publication | Rhodococcus erythropolis | |
| 157.7 | - |
Benzamide | pH and temperature not specified in the publication | Rhodococcus erythropolis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Rhodococcus erythropolis |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | the enzyme is involved in the nitrile hydratase pathway | Rhodococcus erythropolis |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.087 | - |
Acrylamide | pH and temperature not specified in the publication | Rhodococcus erythropolis | |
| 1.14 | - |
acetamide | pH and temperature not specified in the publication | Rhodococcus erythropolis | |
| 4.54 | - |
Propionamide | pH and temperature not specified in the publication | Rhodococcus erythropolis | |
| 153.5 | - |
Benzamide | pH and temperature not specified in the publication | Rhodococcus erythropolis | |
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Release 2023.1 (January 2023)
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