Application | Comment | Organism |
---|---|---|
synthesis | the acyl transfer activity of the enzyme can be used for the synthesis of variety of hydroxamic acids. Optimization of physiochemical parameters result into 30fold increase in the acyl transfer activity of amidase. The acyl transfer activity of amidase of Alcaligenes sp. MTCC 10674 under high temperature condition makes of potential application in developing a bioprocess for the production of variety of aliphatic and aromatic hydroxamic acid | Alcaligenes sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alcaligenes sp. | - |
isolated as a nitrile degrading bacterium from the soil | - |
Alcaligenes sp. MTCC 10674 | - |
isolated as a nitrile degrading bacterium from the soil | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the amidase of the organism exhibits dual activity i.e. hydrolase as well as acyl transfer. The acyl transfer activity of amidase forms an hydroxamate and ammonia from an amide and hydroxylamine and shows broader substrate affinity ranging from a variety of aliphatic amides (formamide, acetamide, propanamide etc.) to aromatic amides (benzamide, mandelamide, nicotinamide etc.) along with hydroxylamine for the biotransformation of these amides into corresponding hydroxamic acid, substrate specificity, overview | Alcaligenes sp. | ? | - |
? | |
additional information | the amidase of the organism exhibits dual activity i.e. hydrolase as well as acyl transfer. The acyl transfer activity of amidase forms an hydroxamate and ammonia from an amide and hydroxylamine and shows broader substrate affinity ranging from a variety of aliphatic amides (formamide, acetamide, propanamide etc.) to aromatic amides (benzamide, mandelamide, nicotinamide etc.) along with hydroxylamine for the biotransformation of these amides into corresponding hydroxamic acid, substrate specificity, overview | Alcaligenes sp. MTCC 10674 | ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 60 | quite stable at 50°C with t1/2 of 8 h, at 60°C t1/2 is 90 min | Alcaligenes sp. |