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Literature summary for 3.5.1.4 extracted from
- The signature amidase from Sulfolobus solfataricus belongs to the CX3C subgroup of enzymes cleaving both amides and nitriles. Ser195 and Cys145 are predicted to be the active site nucleophiles (2005), FEBS J., 272, 4716-4724.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Saccharolobus solfataricus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| K96R | retains the ability to hydrolyse amides but hydrolyses nitriles more efficiently than the wild-type enzyme | Saccharolobus solfataricus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| phenylmethanesulfonyl fluoride | inhibits amide conversion more than 94% and completely suppresses nitrile hydrolysis | Saccharolobus solfataricus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000093 | - |
Propionamide | 85°C, pH not specified in the publication, mutant enzyme K96R | Saccharolobus solfataricus | |
| 0.000099 | - |
Isobutyramide | 85°C, pH not specified in the publication, wild-type enzyme | Saccharolobus solfataricus | |
| 0.000102 | - |
Propionamide | 85°C, pH not specified in the publication, wild-type enzyme | Saccharolobus solfataricus | |
| 0.0002 | - |
Isobutyramide | 85°C, pH not specified in the publication, mutant enzyme K96R | Saccharolobus solfataricus | |
| 0.000434 | - |
cinnamamide | 85°C, pH not specified in the publication, wild-type enzyme | Saccharolobus solfataricus | |
| 0.000526 | - |
cinnamamide | 85°C, pH not specified in the publication, mutant enzyme K96R | Saccharolobus solfataricus | |
| 0.000674 | - |
Benzamide | 85°C, pH not specified in the publication, wild-type enzyme | Saccharolobus solfataricus | |
| 0.000723 | - |
Benzamide | 85°C, pH not specified in the publication, mutant enzyme K96R | Saccharolobus solfataricus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharolobus solfataricus | P95896 | - |
- |
| Saccharolobus solfataricus P2 | P95896 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharolobus solfataricus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.18 | - |
85°C, pH not specified in the publication, substrate: benzamide | Saccharolobus solfataricus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzamide + H2O | - |
Saccharolobus solfataricus | benzoate + NH3 | - |
? | |
| benzamide + H2O | - |
Saccharolobus solfataricus P2 | benzoate + NH3 | - |
? | |
| cinnamamide + H2O | - |
Saccharolobus solfataricus | cinnamic acid + NH3 | - |
? | |
| cinnamamide + H2O | - |
Saccharolobus solfataricus P2 | cinnamic acid + NH3 | - |
? | |
| isobutyramide + H2O | - |
Saccharolobus solfataricus | isobutyrate + NH3 | - |
? | |
| isobutyramide + H2O | - |
Saccharolobus solfataricus P2 | isobutyrate + NH3 | - |
? | |
| propionamide + H2O | - |
Saccharolobus solfataricus | propionate + NH3 | - |
? | |
| propionamide + H2O | - |
Saccharolobus solfataricus P2 | propionate + NH3 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SsAH | - |
Saccharolobus solfataricus |
| SSO2122 | ordered locus name | Saccharolobus solfataricus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
enzyme remains active for about 6 days | Saccharolobus solfataricus |
| 95 | - |
half-life: 5 h | Saccharolobus solfataricus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 160 | - |
Propionamide | 85°C, pH not specified in the publication, mutant enzyme K96R | Saccharolobus solfataricus | |
| 160 | - |
Isobutyramide | 85°C, pH not specified in the publication, mutant enzyme K96R | Saccharolobus solfataricus | |
| 175 | - |
Isobutyramide | 85°C, pH not specified in the publication, wild-type enzyme | Saccharolobus solfataricus | |
| 181 | - |
Propionamide | 85°C, pH not specified in the publication, wild-type enzyme | Saccharolobus solfataricus | |
| 769 | - |
cinnamamide | 85°C, pH not specified in the publication, wild-type enzyme | Saccharolobus solfataricus | |
| 906 | - |
cinnamamide | 85°C, pH not specified in the publication, mutant enzyme K96R | Saccharolobus solfataricus | |
| 1192 | - |
Benzamide | 85°C, pH not specified in the publication, wild-type enzyme | Saccharolobus solfataricus | |
| 1246 | - |
Benzamide | 85°C, pH not specified in the publication, mutant enzyme K96R | Saccharolobus solfataricus | |
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