Cloned (Comment) | Organism |
---|---|
- |
Rhodococcus erythropolis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
HgCl2 | - |
Rhodococcus erythropolis | |
iodoacetamide | - |
Rhodococcus erythropolis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
Benzamide | - |
Rhodococcus erythropolis | |
0.18 | - |
Isobutyramide | - |
Rhodococcus erythropolis | |
0.18 | - |
butyramide | - |
Rhodococcus erythropolis | |
1.3 | - |
Propionamide | - |
Rhodococcus erythropolis | |
4.79 | - |
Acrylamide | - |
Rhodococcus erythropolis | |
5.57 | - |
acetamide | - |
Rhodococcus erythropolis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus erythropolis | B4XEY3 | - |
- |
Rhodococcus erythropolis No. 7 | B4XEY3 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Rhodococcus erythropolis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-chloro-3-hydroxybutyramide + H2O | - |
Rhodococcus erythropolis | 4-chloro-3-hydroxybutyric acid + NH3 | - |
? | |
acetamide + H2O | also acyl transfer activity in the presence of hydroxylamine | Rhodococcus erythropolis | acetic acid + NH3 | - |
? | |
acetamide + H2O | also acyl transfer activity in the presence of hydroxylamine | Rhodococcus erythropolis No. 7 | acetic acid + NH3 | - |
? | |
acrylamide + H2O | also acyl transfer activity in the presence of hydroxylamine | Rhodococcus erythropolis | acrylic acid + NH3 | - |
? | |
acrylamide + H2O | also acyl transfer activity in the presence of hydroxylamine | Rhodococcus erythropolis No. 7 | acrylic acid + NH3 | - |
? | |
benzamide + H2O | also acyl transfer activity in the presence of hydroxylamine | Rhodococcus erythropolis | benzoic acid + NH3 | - |
? | |
benzamide + H2O | also acyl transfer activity in the presence of hydroxylamine | Rhodococcus erythropolis No. 7 | benzoic acid + NH3 | - |
? | |
butyramide + H2O | - |
Rhodococcus erythropolis | butyric acid + NH3 | - |
? | |
isobutyramide + H2O | - |
Rhodococcus erythropolis | isobutyric acid + NH3 | - |
? | |
additional information | enantioselectivity toward (R)-4-chloro-3-hydroxybutyramide: e.e value 52% (conversion yield 57%) | Rhodococcus erythropolis | ? | - |
? | |
additional information | enantioselectivity toward (R)-4-chloro-3-hydroxybutyramide: e.e value 52% (conversion yield 57%) | Rhodococcus erythropolis No. 7 | ? | - |
? | |
propionamide + H2O | also acyl transfer activity in the presence of hydroxylamine | Rhodococcus erythropolis | propionic acid + NH3 | - |
? | |
propionamide + H2O | also acyl transfer activity in the presence of hydroxylamine | Rhodococcus erythropolis No. 7 | propionic acid + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
amidase | - |
Rhodococcus erythropolis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
substrate: isobutyramide, no activity in Tris-HCl buffer | Rhodococcus erythropolis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
up to 50°C, 30 min | Rhodococcus erythropolis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.21 | - |
acetamide | - |
Rhodococcus erythropolis | |
0.48 | - |
Benzamide | - |
Rhodococcus erythropolis | |
0.58 | - |
Acrylamide | - |
Rhodococcus erythropolis | |
2.04 | - |
butyramide | - |
Rhodococcus erythropolis | |
4.03 | - |
Propionamide | - |
Rhodococcus erythropolis | |
5.68 | - |
Isobutyramide | - |
Rhodococcus erythropolis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
substrate: isobutyramide, no activity in Tris-HCl buffer | Rhodococcus erythropolis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 12 | 30 min | Rhodococcus erythropolis |