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Literature summary for 3.5.1.4 extracted from

  • Martins, S.; Karmali, A.; Andrade, J.; Custodio, A.; Serralheiro, M.L.
    Characterization of monoclonal antibodies against altered (T103I) amidase from Pseudomonas aeruginosa (2005), Mol. Biotechnol., 30, 207-219.
    View publication on PubMed
Search for more literature for 3.5.1.4

Protein Variants

Protein Variants Comment Organism
T103I site-directed mutagenesis, binding of specific monoclonal antibodies to the mutant enzyme compared to the wild-type enzyme Pseudomonas aeruginosa

Inhibitors

Inhibitors Comment Organism Structure
additional information effects of specific monoclonal antibodies on wild-type and mutant enzyme activity Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant enzymes by acetamide affinity chromatography and gel filtration Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 effects of specific monoclonal antibodies on wild-type and mutant enzyme activity Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitroacetanilide + H2O
-
 Pseudomonas aeruginosa 4-nitroaniline + acetate
-
 ?

Subunits

Subunits Comment Organism
More epitope binding of specific monoclonal antibodies to the wild-type and T103I mutant enzyme, overview Pseudomonas aeruginosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 assay at Pseudomonas aeruginosa