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Literature summary for 3.5.1.4 extracted from

  • Shaw, N.M.; Naughton, A.B.
    The substrate specificity of the heat-stable stereospecific amidase from Klebsiella oxytoca (2004), Tetrahedron, 60, 747-752.
No PubMed abstract available
Search for more literature for 3.5.1.4

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli strain XL-1 Blue Klebsiella oxytoca

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3,3,3-trifluoro-2-hydroxy-2-methylpropanamide + H2O Klebsiella oxytoca
-
 3,3,3-trifluoro-2-hydroxy-2-methylpropanoic acid + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Klebsiella oxytoca
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme partially from Escherichia coli strain XL-1 Blue by heat treatment of the cell-free extract Klebsiella oxytoca

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 initial reaction rates, partially purified recombinant enzyme Klebsiella oxytoca

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-(-)-2-hydroxy-2-(trifluoromethyl)-butanamide + H2O stereospecific reaction Klebsiella oxytoca (R)-(+)-2-hydroxy-2-(trifluoromethyl)-butanoic acid + NH3 enantiomerically pure product ?
(S)-(-)-3,3,3-trifluoro-2-amino-2-methylpropanamide + H2O stereospecific reaction Klebsiella oxytoca (R)-(+)-3,3,3-trifluoro-2-amino-2-methylpropanoic acid + NH3 enantiomerically pure product ?
(S)-(-)-3,3,3-trifluoro-2-hydroxy-2-methylpropanamide + H2O stereospecific reaction Klebsiella oxytoca (R)-(+)-3,3,3-trifluoro-2-hydroxy-2-methylpropanoic acid + NH3 enantiomerically pure product ?
3,3,3-trifluoro-2-hydroxy-2-methylpropanamide + H2O
-
 Klebsiella oxytoca 3,3,3-trifluoro-2-hydroxy-2-methylpropanoic acid + NH3
-
 ?
additional information substrate synthesis, overview, substrate specificity of the stereospecific enzyme, no activity with 2-hydroxy-2-(trifluoromethyl)-pentanamide, 3,3,3-trifluoro-2-hydroxy-2-phenyl-propionamide, 3,3,3-trichloro-2-hydroxy-2-methylpropionamide, and 3,3,3-trifluoro-2-methoxy-2-methylpropionamide, overview Klebsiella oxytoca ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 assay at Klebsiella oxytoca

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 the enzyme is heat stable Klebsiella oxytoca

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Klebsiella oxytoca