Cloned (Comment) | Organism |
---|---|
gene ST0478, DNA and amino acid sequence determination and analyis, expression in Escherichia coli strain BL21(DE3) as insoluble aggregated His6-tagged protein | Sulfurisphaera tokodaii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
x * 47000, recombinant His6-tagged enzyme, SDS-PAGE | Sulfurisphaera tokodaii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sulfurisphaera tokodaii | - |
gene ST0478 | - |
Sulfurisphaera tokodaii 7 | - |
gene ST0478 | - |
Purification (Comment) | Organism |
---|---|
recombinant insoluble aggregated His6-tagged protein from Escherichia coli by denaturation, refolding during nickel affinity chromatography, gel filtration, and heat treatment, 2.9fold to homogeneity | Sulfurisphaera tokodaii |
Renatured (Comment) | Organism |
---|---|
denaturation and refolding during nickel affinity chromatography of the recombinant insoluble aggregated His6-tagged protein from Escherichia coli | Sulfurisphaera tokodaii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
14.8 | - |
purified recombinant His6-tagged enzyme | Sulfurisphaera tokodaii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R,S)-2-phenylpropionamide + H2O | enantioselective reaction with preference for the S-enantiomer | Sulfurisphaera tokodaii | (S)-2-phenylpropionic acid + (R)-2-phenylpropionamide + NH3 | - |
? | |
(R,S)-2-phenylpropionamide + H2O | enantioselective reaction with preference for the S-enantiomer | Sulfurisphaera tokodaii 7 | (S)-2-phenylpropionic acid + (R)-2-phenylpropionamide + NH3 | - |
? | |
benzamide + H2O | - |
Sulfurisphaera tokodaii | benzoic acid + NH3 | - |
? | |
benzamide + H2O | - |
Sulfurisphaera tokodaii 7 | benzoic acid + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 47000, recombinant His6-tagged enzyme, SDS-PAGE | Sulfurisphaera tokodaii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
about, recombinant enzyme | Sulfurisphaera tokodaii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 80 | 25% of maximal activity at 37°C, maximal activity at 75°C, about 90% of maximal activity at 80°C | Sulfurisphaera tokodaii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
the purified recombinant enzyme is highly thermostable | Sulfurisphaera tokodaii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | recombinant enzyme | Sulfurisphaera tokodaii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 9 | 15% of maximal activity at pH 3.0, 65% at pH 9.0 | Sulfurisphaera tokodaii |