Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Al3+ | 2-linear mixed-type inhibition of the amidase activity | Homo sapiens | |
Ca2+ | 1-linear competitive inhibition of the amidase activity | Homo sapiens | |
K+ | 1-linear competitive inhibition of the amidase activity | Homo sapiens | |
Mg2+ | 1-linear competitive inhibition of the amidase activity | Homo sapiens | |
additional information | cations interact with the active site via Asp189 and the S1 pocket via residue 29, no inhibition by soybean trypsin inhibitor | Homo sapiens | |
Na+ | 1-linear competitive inhibition of the amidase activity | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | urine | Homo sapiens | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
healthy male individuals | - |
Purification (Comment) | Organism |
---|---|
from male urine to homogeneity | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
urine | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Val-L-Leu-L-Arg 4-nitroanilide + H2O | - |
Homo sapiens | D-Val-L-Leu-L-Arg + 4-nitroaniline | - |
? | |
additional information | the human tissue kallikrein shows amidase activity, the enzyme hydrolyzes various synthetic substrates such as Nalpha-substituted arginine and lysine derivatives as esters, amides and fluorogenic peptides, overview | Homo sapiens | ? | - |
? | |
Pro-Phe-Arg benzylamide + H2O | - |
Homo sapiens | Pro-Phe-Arg + benzylamine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
K1 | - |
Homo sapiens |
tissue kallikrein | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
assay at | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics | Homo sapiens | |
2 | - |
Al3+ | pH 9.0, 37°C | Homo sapiens | |
4.9 | - |
Mg2+ | pH 9.0, 37°C | Homo sapiens | |
9.8 | - |
Ca2+ | pH 9.0, 37°C | Homo sapiens | |
22 | - |
Na+ | pH 9.0, 37°C | Homo sapiens | |
67 | - |
K+ | pH 9.0, 37°C | Homo sapiens |