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Literature summary for 3.5.1.4 extracted from
- Cloning, sequence analysis and expression of the gene encoding a novel wide-spectrum amidase belonging to the amidase signature superfamily from Achromobacter xylosoxidans (2005), FEMS Microbiol. Lett., 249, 15-21.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene ana, DNA and amino acid sequence determination and analysis, phylogenetic analysis, the gene might be part of a transposon structure, overexpression of the His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strains DH5alpha and JM109 | Achromobacter xylosoxidans |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.315 | - |
5-[(5-carboxypentanoyl)amino]-2-nitrobenzoic acid | pH 7.5, 37°C | Achromobacter xylosoxidans |  |
| 0.958 | - |
glutaryl-nitro-5-amino benzoic acid | pH 7.5, 37°C | Achromobacter xylosoxidans | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Achromobacter xylosoxidans | Q5YFS2 | gene ana | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to 99% homogeneity | Achromobacter xylosoxidans |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | optimal growth temperature for enzyme production is 26°C | Achromobacter xylosoxidans | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.5 | - |
purified recombinant enzyme, substrate glutaryl-nitro-5-amino benzoic acid | Achromobacter xylosoxidans |
| 4.67 | - |
purified recombinant enzyme, substrate adipoyl-nitro-5-amino benzoic acid | Achromobacter xylosoxidans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-[(4-nitrophenyl)amino]-5-oxopentanoic acid + H2O | - |
Achromobacter xylosoxidans | glutarate + 4-nitroaniline | - |
? | |
| 5-[(5-carboxypentanoyl)amino]-2-nitrobenzoic acid + H2O | - |
Achromobacter xylosoxidans | 2-nitrobenzoate + adipate | - |
? | |
| adipyl-4-nitro-aniline + H2O | - |
Achromobacter xylosoxidans | ? | - |
? | |
| glutaryl-2-naphthylamine + H2O | - |
Achromobacter xylosoxidans | ? | - |
? | |
| glutaryl-aniline + H2O | - |
Achromobacter xylosoxidans | ? | - |
? | |
| glutaryl-nitro-5-amino benzoic acid + H2O | - |
Achromobacter xylosoxidans | ? | - |
? | |
| additional information | substrate specificity towards cephalosporin analogues, overview, no activity with cephalosporin C | Achromobacter xylosoxidans | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| acylase | - |
Achromobacter xylosoxidans |
| Ana | - |
Achromobacter xylosoxidans |
| More | the enzyme belongs to the amidase signature superfamily | Achromobacter xylosoxidans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 44 | - |
- |
Achromobacter xylosoxidans |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | 50 | drastic drop in activity above 50°C, maximal activity at 44°C, assay at 37°C | Achromobacter xylosoxidans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Achromobacter xylosoxidans |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | drastic drop in activity below pH 6.0, maximal activity at pH 8.0 | Achromobacter xylosoxidans |
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