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Literature summary for 3.5.1.4 extracted from

  • Fournand, D.; Arnaud, A.
    Aliphatic and enantioselective amidases: from hydrolysis to acyl transfer activity (2001), J. Appl. Microbiol., 91, 381-393.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
Benzamide acyl transfer reaction on hydroxylamine, pH = 7 Rhodococcus sp.
0.1
-
Isobutyramide acyl transfer reaction on hydroxylamine, pH = 7 Rhodococcus sp.
0.1
-
butyramide acyl transfer reaction on hydroxylamine, pH = 7 Rhodococcus sp.
0.3
-
nicotinamide acyl transfer reaction on hydroxylamine, pH = 7 Rhodococcus sp.
0.3
-
hexanamide acyl transfer reaction on hydroxylamine, pH = 7 Rhodococcus sp.
1
-
Propionamide acyl transfer reaction on hydroxylamine, pH = 7 Rhodococcus sp.
1.3
-
L-Leucinamide acyl transfer reaction on hydroxylamine, pH = 7 Rhodococcus sp.
2
-
L-prolinamide acyl transfer reaction on hydroxylamine, pH = 7 Rhodococcus sp.
3
-
Acrylamide acyl transfer reaction on hydroxylamine, pH = 7 Rhodococcus sp.
5.4
-
L-Methioninamide acyl transfer reaction on hydroxylamine, pH = 7 Rhodococcus sp.
12
-
L-alaninamide acyl transfer reaction on hydroxylamine, pH = 7 Rhodococcus sp.
16
-
L-threoninamide acyl transfer reaction on hydroxylamine, pH = 7 Rhodococcus sp.
70
-
acetamide acyl transfer reaction on hydroxylamine, pH = 7 Rhodococcus sp.

Organism

Organism UniProt Comment Textmining
Cyberlindnera jadinii
-
-
-
Mycobacterium avium
-
-
-
Rhodococcus sp.
-
-
-
Rhodococcus sp. R312
-
-
-
Sus scrofa
-
-
-
Torula cremoris
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetamide + H2O
-
Cyberlindnera jadinii acetate + NH3
-
?
acetamide + H2O
-
Rhodococcus sp. acetate + NH3
-
?
acetamide + H2O
-
Rhodococcus sp. R312 acetate + NH3
-
?
acetamide + hydroxylamine Ping Pong Bi Bi type mechanism Rhodococcus sp. acetylhydroxamic acid + NH3
-
?
acetamide + hydroxylamine Ping Pong Bi Bi type mechanism Rhodococcus sp. R312 acetylhydroxamic acid + NH3
-
?
acrylamide + H2O
-
Cyberlindnera jadinii acrylate + NH3
-
?
acrylamide + hydroxylamine
-
Rhodococcus sp. acrylhydroxamic acid + NH3
-
?
acrylamide + hydroxylamine
-
Rhodococcus sp. R312 acrylhydroxamic acid + NH3
-
?
arachidonoyl-ethanolamine + H2O hydrolysis of anandamide Sus scrofa arachidonic acid + ethanolamine
-
?
benzamide + hydroxylamine
-
Rhodococcus sp. benzoylhydroxamic acid + NH3
-
?
benzamide + hydroxylamine
-
Rhodococcus sp. R312 benzoylhydroxamic acid + NH3
-
?
butyramide + H2O
-
Cyberlindnera jadinii butyrate + NH3
-
?
butyramide + hydroxylamine
-
Rhodococcus sp. butyrylhydroxamic acid + NH3
-
?
butyramide + hydroxylamine
-
Rhodococcus sp. R312 butyrylhydroxamic acid + NH3
-
?
hexanamide + H2O
-
Cyberlindnera jadinii hexanoate + NH3
-
?
hexanamide + hydroxylamine
-
Rhodococcus sp. hexanoylhydroxamic acid + NH3
-
?
isobutyramide + hydroxylamine
-
Rhodococcus sp. isobutyrylhydroxamic acid + NH3
-
?
L-alaninamide + hydroxylamine
-
Rhodococcus sp. L-alaninoylhydroxamic acid + NH3
-
?
L-leucinamide + hydroxylamine
-
Rhodococcus sp. L-leucinoylhydroxamic acid + NH3
-
?
L-methioninamide + hydroxylamine
-
Rhodococcus sp. L-methioninoylhydroxamic acid + NH3
-
?
L-prolinamide + hydroxylamine
-
Rhodococcus sp. L-prolinoylhydroxamic acid + NH3
-
?
L-threoninamide + hydroxylamine
-
Rhodococcus sp. L-threoninoylhydroxamic acid + NH3
-
?
additional information other fatty acid ethanolamides may act as substrate Sus scrofa ?
-
?
nicotinamide + H2O absolute specificity Mycobacterium avium nicotinic acid + NH3
-
?
nicotinamide + H2O absolute specificity Torula cremoris nicotinic acid + NH3
-
?
nicotinamide + hydroxylamine
-
Rhodococcus sp. nicotinoylhydroxamic acid + NH3
-
?
propionamide + H2O
-
Cyberlindnera jadinii propionate + NH3
-
?
propionamide + H2O
-
Rhodococcus sp. propionate + NH3
-
?
propionamide + hydroxylamine
-
Rhodococcus sp. propionylhydroxamic acid + NH3
-
?
short-chain amides + hydrazine
-
Rhodococcus sp. acid hydrazides + NH3
-
?