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Literature summary for 3.5.1.4 extracted from
- Crystallization and X-ray diffraction measurements of a thermophilic archaeal recombinant amidase from Sulfolobus solfataricus MT4 (2001), Acta Crystallogr. Sect. D, D57, 1036-1037.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli of a recombinant His-tagged protein | Saccharolobus solfataricus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| native enzyme using the hanging-drop vapour-diffudion technique, a His-tagged protein cannot be crystallized | Saccharolobus solfataricus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharolobus solfataricus | - |
MT4 strain | - |
| Saccharolobus solfataricus MT4 | - |
MT4 strain | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| extraction of a His-tagged enzyme from insoluble cellular fractions using sodium phosphate buffer at pH 7.8 and further purification by affinity column chromatography | Saccharolobus solfataricus |
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